| Cholesterol diet-induced hyperlipidemia impairs the cardioprotective effect of postconditioning: role of peroxynitrite. | |
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MedLine Citation:
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PMID: 19734363 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the present study was to investigate if hyperlipidemia interferes with the infarct size-limiting effect of postconditioning and to study the involvement of peroxynitrite in this phenomenon. Rats were fed a 2% cholesterol-enriched or normal diet for 12 wk. Infarct size by triphenyltetrazolium chloride staining was measured in hearts isolated from both groups and subjected to 30 min coronary occlusion followed by 120 min reperfusion with or without the postconditioning protocol induced by six cycles of 10 s coronary occlusion and 10 s reperfusion at the onset of the reperfusion. Postconditioning significantly decreased infarct size in the normolipidemic but not in the hyperlipidemic group. Postconditioning increased cardiac 3-nitrotyrosine concentration (a marker for peroxynitrite formation) in the normal but not in the cholesterol-fed group when measured at the 5th min of reperfusion. Next, we tested if the postconditioning-induced acute increase in peroxynitrite is involved in the cardioprotection in normolipidemic animals in separate experiments. Postconditioning failed to decrease infarct size in the presence of the peroxynitrite decomposition catalyst 5,10,15,20-tetrakis-[4-sulfonatophenyl]-porphyrinato-iron [III] (20 mg/l) in normolipidemic animals. We conclude that an early increase in peroxynitrite after postconditioning plays a role in cardioprotection. Furthermore, hyperlipidemia blocks the cardioprotective effect of postconditioning at least in part via deterioration of the postconditioning-induced early increase in peroxynitrite formation. |
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Authors:
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Krisztina Kupai; Csaba Csonka; Veronika Fekete; Louise Odendaal; Jacques van Rooyen; De Wet Marais; Tamás Csont; Péter Ferdinandy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-04 |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 297 ISSN: 1522-1539 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-27 Completed Date: 2009-11-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H1729-35 Citation Subset: IM |
Affiliation:
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Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Hungary. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / metabolism Cholesterol, Dietary / adverse effects Disease Models, Animal Hyperlipidemias / complications*, etiology, metabolism, pathology Male Metalloporphyrins / pharmacology Myocardial Infarction / etiology, metabolism, pathology, prevention & control* Myocardial Reperfusion Injury / etiology, metabolism, pathology, prevention & control* Myocardium / metabolism*, pathology Peroxynitrous Acid / metabolism* Rats Signal Transduction Stress, Physiological* / drug effects Tyrosine / analogs & derivatives, metabolism |
| Chemical | |
Reg. No./Substance:
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0/5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato iron(III) chloride; 0/Biological Markers; 0/Cholesterol, Dietary; 0/Metalloporphyrins; 14691-52-2/Peroxynitrous Acid; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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