Document Detail

Cholesterol diet-induced hyperlipidemia impairs the cardioprotective effect of postconditioning: role of peroxynitrite.
MedLine Citation:
PMID:  19734363     Owner:  NLM     Status:  MEDLINE    
The aim of the present study was to investigate if hyperlipidemia interferes with the infarct size-limiting effect of postconditioning and to study the involvement of peroxynitrite in this phenomenon. Rats were fed a 2% cholesterol-enriched or normal diet for 12 wk. Infarct size by triphenyltetrazolium chloride staining was measured in hearts isolated from both groups and subjected to 30 min coronary occlusion followed by 120 min reperfusion with or without the postconditioning protocol induced by six cycles of 10 s coronary occlusion and 10 s reperfusion at the onset of the reperfusion. Postconditioning significantly decreased infarct size in the normolipidemic but not in the hyperlipidemic group. Postconditioning increased cardiac 3-nitrotyrosine concentration (a marker for peroxynitrite formation) in the normal but not in the cholesterol-fed group when measured at the 5th min of reperfusion. Next, we tested if the postconditioning-induced acute increase in peroxynitrite is involved in the cardioprotection in normolipidemic animals in separate experiments. Postconditioning failed to decrease infarct size in the presence of the peroxynitrite decomposition catalyst 5,10,15,20-tetrakis-[4-sulfonatophenyl]-porphyrinato-iron [III] (20 mg/l) in normolipidemic animals. We conclude that an early increase in peroxynitrite after postconditioning plays a role in cardioprotection. Furthermore, hyperlipidemia blocks the cardioprotective effect of postconditioning at least in part via deterioration of the postconditioning-induced early increase in peroxynitrite formation.
Krisztina Kupai; Csaba Csonka; Veronika Fekete; Louise Odendaal; Jacques van Rooyen; De Wet Marais; Tamás Csont; Péter Ferdinandy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-04
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  297     ISSN:  1522-1539     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-27     Completed Date:  2009-11-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1729-35     Citation Subset:  IM    
Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Hungary.
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MeSH Terms
Biological Markers / metabolism
Cholesterol, Dietary / adverse effects
Disease Models, Animal
Hyperlipidemias / complications*,  etiology,  metabolism,  pathology
Metalloporphyrins / pharmacology
Myocardial Infarction / etiology,  metabolism,  pathology,  prevention & control*
Myocardial Reperfusion Injury / etiology,  metabolism,  pathology,  prevention & control*
Myocardium / metabolism*,  pathology
Peroxynitrous Acid / metabolism*
Signal Transduction
Stress, Physiological* / drug effects
Tyrosine / analogs & derivatives,  metabolism
Reg. No./Substance:
0/5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato iron(III) chloride; 0/Biological Markers; 0/Cholesterol, Dietary; 0/Metalloporphyrins; 14691-52-2/Peroxynitrous Acid; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine

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