| Cholesterol degradation by Gordonia cholesterolivorans. | |
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MedLine Citation:
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PMID: 21622796 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This paper reports physiological and genetic data about the type strain Gordonia cholesterolivorans, a strain that is able to degrade steroid compounds containing a long carbon side chain such as cholesterol (C27), cholestenone (C27), ergosterol (C28) and stigmasterol (C29). The length of the carbon side chain appears to be of great importance for this bacterium, as the strain is unable to grow using steroids with a shorter or none aliphatic carbon side chain such as cholic acid (C24), progesterone (C21), testosterone, androsterone, 4-androstene-3,17-dione (all C19) and further steroids. This study also demonstrates that the degradation of cholesterol is a quite common feature of the genus Gordonia (G.) by comparing G. cholesterolivorans with some other species of this genus (e.g., G. sihwensis, G. hydrophobica, G. australis and G. neofelifaecis). Pyrosequencing of the genome of G. cholesterolivorans led to the identification of two conventional cholesterol oxidase genes on a 8 kb and a 12.8 kb genomic fragment with genetic organizations that are quite unique as compared to the genomes of other cholesterol-degrading bacteria so far. The identified two putative cholesterol oxidases of G. cholesterolivorans are both intracellular-acting enzymes of the class I type. Whereas one of these two cholesterol oxidases (ChoOx-1) shows high identity with an oxidoreductase of the opportunistic pathogen G. bronchialis and is not transcribed during growth with cholesterol, the other one (ChoOx-2) appears phylogenetically closer to cholesterol oxidases from members of the genus Rhodococcus and is transcribed constitutively. By using targeted gene disruption, a G. cholesterolivorans choox-2 mutant strain was obtained that was unable to grow with steroids. |
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Authors:
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O Drzyzga; L Fernández de Las Heras; V Morales; J M Navarro Llorens; J Perera |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-27 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: - ISSN: 1098-5336 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid, 28040 Madrid, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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