| Cholesterol conservation in skeletal muscle associated with age- and denervation-related atrophy. | |
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MedLine Citation:
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PMID: 2776002 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lipid composition of muscles with age- and denervation-atrophy was assayed in whole muscle and isolated sarcolemma of rats aged 10 and 25 months. Although muscle mass decreased at least 17% during aging, muscle cholesterol concentration rose 15-20%. However, phospholipid concentrations were maintained; therefore, the cholesterol-to-phospholipid ratio increased during aging. Plasma cholesterol levels also rose 35%, but this could account for only about 10% of the age-related difference in muscle cholesterol. Likewise, following denervation (7 days) muscle mass decreased by at least 30%, but muscle cholesterol-to-phospholipid ratio increased; the magnitude of the denervation-induced response was unaffected by aging. To localize the source of cholesterol deposition during aging, these assays were repeated on isolated sarcolemma, sarcoplasmic reticulum, and mitochondria membrane fractions. Neither cholesterol nor phospholipid levels changed significantly with age in any of these major membrane systems. Furthermore, sarcolemmal fluidity, which depends on cholesterol content, did not vary significantly with age. At this level of resolution, it thus appears that skeletal muscle membrane phospholipid composition and fluidity do not change appreciably with age. Elevated cholesterol-to-phospholipid ratios detected in whole muscle may be due to cholesterol deposition in non-myofiber locations. |
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Authors:
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K D Williams; D O Smith |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research Volume: 493 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1989 Jul |
Date Detail:
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Created Date: 1989-10-25 Completed Date: 1989-10-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 14-22 Citation Subset: IM; S |
Affiliation:
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Department of Physiology, University of Wisconsin, Madison 53706. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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metabolism* Animals Cell Membrane / metabolism Cholesterol / blood, metabolism* Intracellular Membranes / metabolism Male Membrane Fluidity Mitochondria / metabolism Muscle Denervation* Muscles / metabolism*, pathology Muscular Atrophy / etiology, metabolism*, pathology Organ Size Phospholipids / metabolism Rats Rats, Inbred F344 Sarcolemma / metabolism Sarcoplasmic Reticulum / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AG01572/AG/NIA NIH HHS; AG05427/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phospholipids; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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