| Cholesterol changes the fatty acid composition of rat enterocytes. | |
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MedLine Citation:
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PMID: 12532238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of free cholesterol on the fatty acid composition and growth of rat fetal enterocytes was investigated in the absence and presence of 10% (v/v) fetal calf serum. Cholesterol caused a significant reduction of cell number after 6 and 12 h in culture. The fatty acid composition of enterocytes cultured in the presence of serum was also changed by the presence of 20 microM cholesterol. The fatty acid profile was determined by HPLC using fluorescence detection (325 nm excitation and 395 nm emission). Cholesterol (20 microM) increased the proportion (given in percentage of the total fatty acids) of the following fatty acids in cultured cells: lauric (by 42%), oleic (by 34%), linoleic (by 44%) and gamma-linolenic (by 20%) acids and reduced the proportion of palmitic (by 12%), stearic (by 20%), arachidonic (by 21%) and docosahexaenoic (by 44%) acids. In addition to modifying the content of individual fatty acids, cholesterol increased the polyunsaturated/saturated fatty acid ratio from 0.48 to 0.67 and the unsaturation index from 67.12 to 75.30. This is the first evidence that cholesterol modifies fatty acid composition possibly via de novo fatty acid synthesis and desaturation. |
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Authors:
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J Gazzola; E F Martins; C K Miyasaka; A C Palanch; M G Vecchia; R Curi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2002-12-19 |
Journal Detail:
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Title: Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.] Volume: 36 ISSN: 0100-879X ISO Abbreviation: Braz. J. Med. Biol. Res. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-01-17 Completed Date: 2003-05-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8112917 Medline TA: Braz J Med Biol Res Country: Brazil |
Other Details:
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Languages: eng Pagination: 137-41 Citation Subset: IM |
Affiliation:
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Departamento de Ciências da Saúde, Universidade de Ijuí, Ijuí, RS, Brasil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division Cholesterol / metabolism* Chromatography, High Pressure Liquid Enterocytes / chemistry*, physiology Fatty Acids / chemistry*, metabolism Female Male Pregnancy Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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