| Cholesterol absorption and metabolism. | |
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MedLine Citation:
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PMID: 20012398 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inhibitors of cholesterol absorption have been sought for decades as a means to treat and prevent cardiovascular diseases associated with hypercholesterolemia. Ezetimibe is the one clear success story in this regard, and other compounds with similar efficacy continue to be sought. In the last decade, the laboratory mouse, with all its genetic power, has become the premier experimental model for discovering the mechanisms underlying cholesterol absorption and has become a critical tool for preclinical testing of potential pharmaceutical entities. This chapter briefly reviews the history of cholesterol absorption research and the various gene candidates that have come under consideration as drug targets. The most common and versatile method of measuring cholesterol absorption is described in detail along with important considerations when interpreting results, and an alternative method is also presented. In recent years, reverse cholesterol transport has become an area of intense new interest for drug discovery since this process is now considered another key to reducing cardiovascular disease risk. The ultimate measure of reverse cholesterol transport is sterol excretion and a detailed description is given for measuring neutral and acidic fecal sterols and interpreting the results. |
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Authors:
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Philip N Howles |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 602 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2009-12-16 Completed Date: 2010-02-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 157-79 Citation Subset: IM |
Affiliation:
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Department of Pathology, Center for Lipid and Arteriosclerosis Studies, Genome Research Institute, University of Cincinnati College of Medicine, Cincinnati, OH, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Radioisotopes / metabolism Cardiovascular Diseases / drug therapy, etiology*, metabolism Cholesterol / chemistry, metabolism* Diet Drug Discovery Feces / chemistry Humans Hypercholesterolemia / complications*, drug therapy, metabolism* Intestinal Absorption / physiology* Mice Mice, Inbred C57BL Molecular Structure Tritium / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 10028-17-8/Tritium; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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