| Cholestenoic Acid is an important elimination product of cholesterol in the retina: comparison of retinal cholesterol metabolism with that in the brain. | |
| | |
MedLine Citation:
|
PMID: 20881306 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: Accumulating evidence indicates a link between cholesterol and age-related macular degeneration. Yet, little is known about cholesterol elimination from the retina and retinal pigment epithelium (RPE), the two layers that are damaged in this blinding disease. Several different pathways of enzymatic cholesterol removal exist in extraocular tissues. The authors tested whether metabolites from these pathways could also be quantified in the bovine and human retina and RPE. For comparison, they measured cholesterol oxidation products in two regions of the bovine and human brain and in the bovine liver and adrenal glands. METHODS: Sterol quantification was carried out by isotope dilution gas chromatography-mass spectrometry. Bovine tissues were used first to optimize analytical procedures and to investigate postmortem changes in oxysterol concentrations. Then human specimens were analyzed for oxysterol concentrations. RESULTS: Qualitatively, oxysterol profiles were similar in the bovine and human tissues. In the human retina and RPE, the authors could not detect 27-hydroxycholesterol but unexpectedly found that its oxidation product, 5-cholestenoic acid, is the most abundant oxysterol, varying up to threefold in different persons. 24S-Hydroxysterol and pregnenolone were also present in the retina, but at much lower quantities and without significant interindividual variability. In the brain, the predominant oxysterol was 24S-hydroxycholesterol. CONCLUSIONS: The oxysterol profile of the retina suggests that all known pathways of cholesterol elimination in extraocular organs are operative in the retina and that they likely vary depending on specific cell type. However, overall oxidation to 5-cholestenoic acid appears to be the predominant mechanism for cholesterol elimination from this organ. |
| | |
Authors:
|
Natalia Mast; Rachel Reem; Ilya Bederman; Suber Huang; Pier Luigi DiPatre; Ingemar Bjorkhem; Irina A Pikuleva |
Related Documents
:
|
145876 - Effect of liposomes containing cholesterol on adenylate cyclase activity of cultured ma... 18787236 - Serum, but not monocyte macrophage foam cells derived from low hdl-c subjects, displays... 20491656 - Overexpression of stard3 in human monocyte/macrophages induces an anti-atherogenic lipi... 21053746 - An agonist of liver x receptor slows valvular disease in a hypercholesterolemia mouse m... 16713156 - Antihypercholesterolaemic and antioxidant activity assessment of some plants used as re... 15972076 - Effects of long-term growth hormone-releasing factor treatment on growth, feed conversi... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-01 |
Journal Detail:
|
Title: Investigative ophthalmology & visual science Volume: 52 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-02-02 Completed Date: 2011-03-09 Revised Date: 2012-05-24 |
Medline Journal Info:
|
Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 594-603 Citation Subset: IM |
Affiliation:
|
Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, OH 44106, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenal Glands
/
metabolism Aged Aged, 80 and over Animals Brain / metabolism* Cattle Cholesterol / analogs & derivatives*, metabolism* Cholesterol Side-Chain Cleavage Enzyme / metabolism Cytochrome P-450 CYP27A1 / metabolism Female Gas Chromatography-Mass Spectrometry Humans Hydroxycholesterols / metabolism Liver / metabolism Male Middle Aged Retina / metabolism* Retinal Pigment Epithelium / metabolism Steroid Hydroxylases / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
AG024336/AG/NIA NIH HHS; EY018383/EY/NEI NIH HHS; T32 EY007157/EY/NEI NIH HHS; T32 EY007157-09/EY/NEI NIH HHS; T32 EY007157-10/EY/NEI NIH HHS; T32 EY07157/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Hydroxycholesterols; 474-73-7/24-hydroxycholesterol; 57-88-5/Cholesterol; 6561-58-6/3-hydroxy-5-cholestenoic acid; EC 1.14.-/Cytochrome P-450 CYP27A1; EC 1.14.-/Steroid Hydroxylases; EC 1.14.-/cholesterol 24-hydroxylase; EC 1.14.15.6/Cholesterol Side-Chain Cleavage Enzyme |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of artesunate on endotoxin-induced uveitis in rats.
Next Document: Dynamic changes of microRNAs in the eye during the development of experimental autoimmune uveoretini...