| Cholangiocyte bile salt transporters in cholesterol gallstone-susceptible and resistant inbred mouse strains. | |
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MedLine Citation:
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PMID: 18717763 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND AIM: We investigated the dietary and gender influences on the expression and functionality of cholangiocyte bile salt transporters and development of biliary hyperplasia in cholesterol gallstone-susceptible C57L/J and resistant AKR/J mice. METHODS: C57L and AKR mice were fed chow, a lithogenic diet, or a cholic acid-containing diet for 14 days. Expression of cholangiocyte bile salt transporter proteins ASBT (SLC10A2), ILBP (FABP6), and MRP3 (ABCC3) were studied by Western blot analysis. Taurocholate uptake studies were performed using microperfusion of isolated bile duct units. The pre- and post-perfusion taurocholate concentrations were analyzed by high performance liquid chromatography. Biliary proliferation in liver sections was scored. RESULTS: The lithogenic diet induced ductular proliferation in C57L mice. On chow, SLC10A2 and ABCC3 were overexpressed in male and female C57L compared to AKR mice. A lithogenic diet reduced the expressions of FABP6 in both male and female C57L mice, SLC10A2 in female C57L mice, and ABCC3 in male C57L mice. These alterations in transporter expressions were not associated with changes in taurocholate uptake. The lithogenic diet induced biliary hyperplasia and reduced bile salt transporter expressions in C57L mice. CONCLUSIONS: Although bile salt uptake was not increased in the bile duct unit, we speculate that the biliary hyperplasia on the lithogenic diet may lead to an increase in intrahepatic bile salt recycling during cholesterol cholelithogenesis. |
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Authors:
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Julia J Liu; Jonathan N Glickman; Anatoliy I Masyuk; Nicholas F Larusso |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-08-20 |
Journal Detail:
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Title: Journal of gastroenterology and hepatology Volume: 23 ISSN: 1440-1746 ISO Abbreviation: J. Gastroenterol. Hepatol. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2009-01-05 Completed Date: 2009-04-09 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 8607909 Medline TA: J Gastroenterol Hepatol Country: Australia |
Other Details:
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Languages: eng Pagination: 1596-602 Citation Subset: IM |
Affiliation:
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Division of Gastroenterology, Department of Medicine, University of Alberta Hospital, Edmonton, Alberta, Canada. julia.liu@ualberta.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile Ducts / metabolism*, pathology Cell Proliferation Cholesterol, Dietary Disease Models, Animal Disease Susceptibility Fatty Acid-Binding Proteins / metabolism Female Gallstones / etiology, metabolism*, pathology Gastrointestinal Hormones / metabolism Hyperplasia Male Membrane Transport Proteins / metabolism* Mice Mice, Inbred AKR Mice, Inbred C57BL Multidrug Resistance-Associated Proteins / metabolism Organic Anion Transporters, Sodium-Dependent / metabolism Sex Factors Species Specificity Symporters / metabolism Taurocholic Acid / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DK 24031/DK/NIDDK NIH HHS; DK 36588/DK/NIDDK NIH HHS; DK 52911/DK/NIDDK NIH HHS; DK 57993/DK/NIDDK NIH HHS; R01 DK024031-31/DK/NIDDK NIH HHS; R01 DK057993-07/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cholesterol, Dietary; 0/Fatty Acid-Binding Proteins; 0/Gastrointestinal Hormones; 0/Membrane Transport Proteins; 0/Multidrug Resistance-Associated Proteins; 0/Organic Anion Transporters, Sodium-Dependent; 0/Symporters; 0/multidrug resistance-associated protein 3; 117849-44-2/fatty acid-binding protein 6; 145420-23-1/sodium-bile acid cotransporter; 81-24-3/Taurocholic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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