Document Detail

Choice of infusion-sampling mode for tracer studies of free fatty acid metabolism.
MedLine Citation:
PMID:  3364559     Owner:  NLM     Status:  MEDLINE    
To determine the preferred infusion-sampling mode for isotopic studies of free fatty acid (FFA) metabolism, tracer [( 14C]palmitate) was infused into the left ventricle of five anesthetized dogs, and tracee ([3H]palmitate) was infused into three separate peripheral veins of each dog. The [14C]palmitate specific activity (SA) was lower in mixed venous than arterial blood, and [3H]palmitate SA was equal in both sites. The actual infusion rate of [3H]palmitate [2.15 +/- 0.31 X 10(5) disintegrations/min (dpm).kg-1.min-1] could be accurately predicted (2.14 +/- 0.32 X 10(5) using the known [14C]palmitate infusion rate and the arterial plasma [14C]-to-[3H]palmitate ratio. In contrast, the mixed venous [14C]-to-[3H]palmitate ratio resulted in overestimates (P less than 0.05) of the actual [3H]palmitate infusion rate. In summary, venous tracer infusion with arterial blood sampling for FFA tracer studies provides the most accurate estimates of tracee rate of appearance.
M D Jensen; P J Rogers; M G Ellman; J M Miles
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  254     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-06-09     Completed Date:  1988-06-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E562-5     Citation Subset:  IM    
Department of Internal Medicine, Mayo Medical School, Rochester, Minnesota 55905.
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MeSH Terms
Carbon Radioisotopes
Chromatography, High Pressure Liquid
Fatty Acids, Nonesterified / blood*
Palmitic Acid
Palmitic Acids / administration & dosage,  blood
Radioactive Tracers / administration & dosage*
Radioisotopes / administration & dosage*
Grant Support
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Fatty Acids, Nonesterified; 0/Palmitic Acids; 0/Radioactive Tracers; 0/Radioisotopes; 10028-17-8/Tritium; 57-10-3/Palmitic Acid
Comment In:
Am J Physiol. 1989 Feb;256(2 Pt 1):E336-7   [PMID:  2919671 ]

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