Document Detail

Choice of Pore Size Can Introduce Artefacts when Filtering Picoeukaryotes for Molecular Biodiversity Studies.
MedLine Citation:
PMID:  23325466     Owner:  NLM     Status:  Publisher    
Published results of studies based on samples size fractionated by sequential filtration (e.g. 0.2-3 μm) indicate that many ciliate, dinoflagellate and rhizarian phylotypes are found among marine picoeukaryotes. This is somewhat surprising as these protists are typically known as being large organisms (often >10 μm) and no picoplanktonic species have so far been identified. Here, the abundances of ciliate and dinoflagellate phylotypes in published molecular studies of picoeukaryotes are shown to correlate negatively with the pore size chosen for the end filter in the sequential filtrations (i.e. the filter used to collect the microbial biomass). This suggests that extracellular DNA adhering to small particles may be the source of ciliate and dinoflagellate phylotypes in picoplanktonic size fractions. This hypothesis was confirmed using real-time qPCR, which revealed significantly less dinoflagellate 18S rDNA in a 0.8-3-μm size fraction compared to 0.2-3 μm. On average, the abundance of putative extracellular phylotypes decreased by 84-89 % when a 0.8- μm end filter was used rather than a 0.2-μm end filter. A 0.8-μm filter is, however, not sufficient to retain all picoeukaryotic cells. Thus, selection of filter pore size involves a trade-off between avoiding artefacts generated by extracellular DNA and sampling the entire picoeukaryotic community. In contrast to ciliate and dinoflagellate phylotypes, rhizarian phylotypes in the picoplankton size range do not display a pattern consistent with an extracellular origin. This is likely due to the documented existence of picoplanktonic swarmer cells within this group.
Nikolaj Sørensen; Niels Daugbjerg; Katherine Richardson
Related Documents :
25340636 - A closed parameterization of dna-damage by charged particles, as a function of energy -...
2295126 - Nitrogen dioxide induces dna single-strand breaks in cultured chinese hamster cells.
11751826 - Localization of uvra and effect of dna damage on the chromosome of bacillus subtilis.
20049126 - A novel approach using dna-repair-deficient chicken dt40 cell lines for screening and c...
16372196 - Layered double hydroxide minerals as possible prebiotic information storage and transfe...
12734566 - Pcr-based detection of a rare linear dna in cell culture.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-17
Journal Detail:
Title:  Microbial ecology     Volume:  -     ISSN:  1432-184X     ISO Abbreviation:  Microb. Ecol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7500663     Medline TA:  Microb Ecol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Center for Macroecology, Evolution and Climate, University of Copenhagen, Universitetsparken 15, 2100, Copenhagen, Denmark,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Changing Views of the Interconnections Between the Oceans and Human Health in Europe.
Next Document:  Regulation of muscle acetylcholine receptor turnover by ? subunit tyrosine phosphorylation.