| Chloroplastic oxidative burst induced by tenuazonic acid, a natural photosynthesis inhibitor, triggers cell necrosis in Eupatorium adenophorum Spreng. | |
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MedLine Citation:
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PMID: 20026008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tenuazonic acid (TeA), a nonhost-specific phytotoxin produced by Alternaria alternata, was determined to be a novel natural photosynthesis inhibitor owning several action sites in chloroplasts. To further elucidate the mode of its action, studies were conducted to assess the production and involvement of reactive oxygen species (ROS) in the toxic activity of TeA. A series of experiments indicated that TeA treatment can induce chloroplast-derived ROS generation including not only (1)O(2) but also superoxide radical, H(2)O(2) and hydroxyl radicals in Eupatorium adenophorum mesophyll cells, resulting from electron leakage and charge recombination in PSII as well as thylakoid overenergization due to inhibition of the PSII electron transport beyond Q(A) and the reduction of end acceptors on the PSI acceptor side and chloroplast ATPase activity. The initial production of TeA-induced ROS was restricted to chloroplasts and accompanied with a certain degree of chloroplast damage. Subsequently, abundant ROS were quickly dispersed throughout whole cell and cellular compartments, causing a series of irreversible cellular harm such as chlorophyll breakdown, lipid peroxidation, plasma membrane rupture, chromatin condensation, DNA cleavage, and organelle disintegration, and finally resulting in rapid cell destruction and leaf necrosis. These results show that TeA causing cell necrosis of host-plants is a result of direct oxidative damage from chloroplast-mediated ROS eruption. |
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Authors:
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Shiguo Chen; Chunyan Yin; Sheng Qiang; Fenyan Zhou; Xinbin Dai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-21 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1797 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-09 Completed Date: 2010-04-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 391-405 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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Weed Research Laboratory, Nanjing Agricultural University, Nanjing 210095, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ageratina
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drug effects* Antibiotics, Antineoplastic / pharmacology* Chloroplasts / chemistry* Chromatin / metabolism DNA Fragmentation Electron Spin Resonance Spectroscopy Hydrogen Peroxide / pharmacology Hydroxyl Radical / metabolism Lipid Peroxidation Necrosis Oxidation-Reduction Oxidative Stress Photosynthesis / drug effects* Photosystem II Protein Complex / metabolism Plant Leaves / drug effects, metabolism Reactive Oxygen Species / metabolism Respiratory Burst* Superoxides / metabolism Tenuazonic Acid / pharmacology* Thylakoids / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Chromatin; 0/Photosystem II Protein Complex; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 3352-57-6/Hydroxyl Radical; 610-88-8/Tenuazonic Acid; 7722-84-1/Hydrogen Peroxide |
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