Document Detail

Chloroplast DNA base substitutions: an experimental assessment.
MedLine Citation:
PMID:  15744500     Owner:  NLM     Status:  MEDLINE    
An experimental assessment was carried out to determine directly the frequency and types of spontaneous base substitutions that occur in chloroplast DNA. A target site within the chloroplast 16S rRNA gene of the green alga Chlamydomonas reinhardtii was chosen for the assay. Mutations at this site were known to confer spectinomycin resistance and simultaneously result in the loss of an AatII cleavage site. In the experiments reported here, base substitutions at any individual base occurred at a frequency in the range of 0.9-11 per 10(9) viable cells plated. Four new mutations that confer resistance to spectinomycin were identified at the target site in the Chlamydomonas chloroplast 16S rRNA gene. When the relative rates of transition and transversion mutations were quantified, a bias toward transversions was observed. The prominence of A/T --> C/G transversions in the observed mutation spectrum suggests that oxidative damage may be the major cause of base substitution mutations within the chloroplast.
Monica Guhamajumdar; Barbara B Sears
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2005-03-03
Journal Detail:
Title:  Molecular genetics and genomics : MGG     Volume:  273     ISSN:  1617-4615     ISO Abbreviation:  Mol. Genet. Genomics     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-27     Completed Date:  2005-07-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101093320     Medline TA:  Mol Genet Genomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  177-83     Citation Subset:  IM    
Department of Plant Biology, Michigan State University, East Lansing, MI, 48824-1312, USA.
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MeSH Terms
Base Sequence
Chlamydomonas reinhardtii / genetics*
DNA Primers
DNA, Chloroplast / genetics*
Drug Resistance / genetics*
Point Mutation / genetics*
RNA, Ribosomal, 16S / genetics*
Sequence Analysis, DNA
Reg. No./Substance:
0/DNA Primers; 0/DNA, Chloroplast; 0/RNA, Ribosomal, 16S; 1695-77-8/Spectinomycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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