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Chlorophyll a Phytylation is Required for the Stability of Photosystems I and II in the Cyanobacterium Synechocystis sp. PCC 6803.
MedLine Citation:
PMID:  23039123     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In oxygenic phototrophic organisms, the phytyl 'tail' of chlorophyll a is formed from a geranylgeranyl residue by the enzyme geranylgeranyl reductase. Additionally, in oxygenic phototrophs phytyl residues are the tail moieties of tocopherols and phylloquinone. A mutant of the cyanobacterium Synechocystis sp. PCC 6803 lacking geranylgeranyl reductase, ΔchlP, was compared to strains with specific deficiencies in either tocopherols or phylloquinone to assess the role of chlorophyll a phytylatation (vs. geranylgeranylation). The tocopherol-less Δhpt strain grows indistinguishably from the wild type under 'standard' light photoautotrophic conditions and exhibited only a slightly enhanced rate of photosystem I degradation under strong irradiation. The phylloquinone-less ΔmenA mutant also grows photoautotrophically, albeit rather slowly and only at low light intensities. Under strong irradiation, ΔmenA retained its chlorophyll content indicative of stable photosystems. Discernibly, ΔchlP can be cultured only photomixotrophically (due to instability of both, photosystems I and II). Increased accumulation of myxoxanthophyll in ΔchlP cells indicates photo-oxidative stress even under moderate illumination. Under high light conditions, ΔchlP exhibited rapidly degrading photosystems I and II. In conclusion, the results demonstrate that chlorophyll a phytylation is important for (photo)stability of photosystems I and II, which, in turn, is necessary for photoautotrophic growth and tolerance of high light in an oxygenic environment. © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Authors:
Alexey V Shpilyov; Vladislav V Zinchenko; Bernhard Grimm; Heiko Lokstein
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-8
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  -     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Affiliation:
Genetics Department, Biology Division, Lomonosov Moscow State University, Moscow, 119899, Russia; Institut für Biologie/Pflanzenphysiologie, Humboldt-Universität zu Berlin, Unter den Linden 6 (Sitz: Philippstr.13), D-10099, Berlin, Germany.
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