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Chlorophyll determination in intact tissues using n,n-dimethylformamide.
MedLine Citation:
PMID:  16661217     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Photosynthetic pigments from etiolated cucumber (Cucumis sativus var. Beit Alpha improved, Hazera Co., Gedera) cotyledons were extracted by direct immersion of the intact cotyledons into the solvent N,N-dimethylformamide (DMF). The solvent is especially efficient when pigment concentration is low; time and tools are saved and the loss of pigment that usually occurs in more complicated extraction procedures is prevented. The specific absorption coefficient of chlorophyll a in DMF was also determined.
Authors:
R Moran; D Porath
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  65     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1980 Mar 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  478-9     Citation Subset:  -    
Affiliation:
Department of Botany, The George S. Wise Faculty for Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
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