| Chlorophenols and chlorocatechols induce apoptosis in human lymphocytes (in vitro). | |
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MedLine Citation:
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PMID: 19766705 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this work the effect of 2,4,5-trichlorophenol (2,4,5-TCP), pentachlorophenol (PCP), 4,6-dichloroguaiacol (4,6-DCG), tetrachloroguaiacol (TeCG), 4,5-dichlorocatechol (4,5-DCC) and tetrachlorocatechol (TeCC) on the induction of apoptosis in human peripheral blood lymphocytes was examined. The analysis of the changes in mitochondrial transmembrane potential (DeltaPsi(m)) was performed using JC-9 fluorescent probe. It was noted that all the compounds studied (excluding 4,6-DCG) in a dose-dependent manner (0.2-25 ppm) increased the number of cells, which were characterized by the DeltaPsi(m) reduction, with the strongest effect observed for TeCG and TeCC. Moreover, fluorimetric assay of the caspase-3 activity revealed that 2,4,5-TCP, chlorocatechols and TeCG in the range of the concentrations of 1-50 ppm increased the caspase-3 expression, whereas PCP in its highest concentration of 50 ppm decreased the activity of this enzyme. It was also noted that the caspase-3 activity was the most strongly induced by tetrachlorocatechols, particularly by TeCC. The analysis of YO-PRO-1 iodide/propidum iodide staining revealed that TeCC, the most strongly increased the number of apoptotic cells at 5 and 25 ppm, whereas at a dose of 100 ppm the strongest effect on apoptosis induction was observed for 2,4,5-TCP. |
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Authors:
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Jaromir Michałowicz; Paulina Sicińska |
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Publication Detail:
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Type: In Vitro; Journal Article Date: 2009-09-18 |
Journal Detail:
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Title: Toxicology letters Volume: 191 ISSN: 1879-3169 ISO Abbreviation: Toxicol. Lett. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-20 Completed Date: 2009-12-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 246-52 Citation Subset: IM |
Affiliation:
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Department of Environmental Pollution Biophysics, University of Łódź, Banacha Str. 12/16, 90-237 Łódź, Poland. michalow@biol.uni.lodz.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Caspase 3 / antagonists & inhibitors, biosynthesis, metabolism Catechols / toxicity* Cell Membrane Permeability / drug effects Cell Separation Chlorophenols / toxicity* Coloring Agents Humans Lymphocytes / drug effects*, enzymology Membrane Potentials / drug effects Mitochondrial Membranes / drug effects Propidium |
| Chemical | |
Reg. No./Substance:
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0/Catechols; 0/Chlorophenols; 0/Coloring Agents; 36015-30-2/Propidium; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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