| Chloroacetonitrile induces oxidative stress and apoptosis in mouse fetal liver. | |
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MedLine Citation:
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PMID: 19576970 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chloroacetonitrile (CAN) is a disinfection by-product of chlorination of drinking water. The present study was designed to investigate the potential adverse effects of maternal exposure to CAN on fetal liver in mice. Based on an initial dose-response experiment, CAN (25mg/(kgday)) was given orally to pregnant mice at gestation day (GD) 6, till GD 18. Fetuses were collected and fetal livers were used for assessing oxidative status, apoptosis and histopathological changes. Maternal exposure to CAN resulted in observed oxidative stress and redox imbalance in fetal liver tissues as marked by significant decrease in reduced glutathione (GSH) and elevation of oxidized glutathione (GSSG), malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in genomic DNA. Further, CAN induced apoptosis as indicated by a significant increase in binding of Hoechst reagent to damaged DNA fragments of fetal liver and enhancement of the activity of caspase-3 in cytosolic fractions of fetal livers. Histopathological examination of fetal livers of CAN-treated mice showed hepatocytes with vacuolated cytoplasm, karyolysis and karyorrhexis as well as depletion of their glycogen content. In conclusion, maternal exposure to CAN adversely affects mouse fetal livers as evidenced by the induction of oxidative stress, apoptosis and histopathological changes. |
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Authors:
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Ashraf B Abdel-Naim; Ayman A Nagy; Ahmed M Mohamadin; Hala M El-Mazar; Ahmed E Ahmed |
Publication Detail:
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Type: Journal Article Date: 2009-07-02 |
Journal Detail:
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Title: Toxicology letters Volume: 190 ISSN: 1879-3169 ISO Abbreviation: Toxicol. Lett. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-07 Completed Date: 2009-09-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 123-7 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetonitriles
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toxicity* Animals Apoptosis / drug effects* Body Weight / drug effects Caspase 3 / biosynthesis DNA Fragmentation / drug effects Deoxyguanosine / analogs & derivatives, metabolism Female Fetus / metabolism, pathology Glutathione / metabolism Liver / embryology, metabolism, pathology* Malondialdehyde / metabolism Mice Oxidation-Reduction Oxidative Stress / drug effects* Pregnancy Survival Water Pollutants, Chemical / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/8-hydroxy-2'-deoxyguanosine; 0/Acetonitriles; 0/Water Pollutants, Chemical; 107-14-2/chloroacetonitrile; 542-78-9/Malondialdehyde; 70-18-8/Glutathione; 961-07-9/Deoxyguanosine; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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