| Chlorination effect on the fluorescence of nucleic acid staining dyes. | |
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MedLine Citation:
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PMID: 15350425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An alternative to culture methods for the control of drinking water disinfection would use fluorescent dyes that could evidence the nucleic acid damages provoked by sodium hypochlorite treatment. The two dyes selected in this study, SYBR Green II RNA gel stain and TOTO-1 iodide, efficiently stain nucleic acids (DNA and RNA) and quite poorly the other biomolecules considered (Bovine serum albumin, palmitic acid and dextrane). After treatment of nucleic acid solutions with increasing amounts of sodium hypochlorite, a decrease of fluorescence intensity is observed for both DNA and RNA stained with either SYBR-II or TOTO-1. However, the two fluorochromes do not lead to the same results, which shows that the two dyes are not bound to nucleic acids in the same way. Contrary to TOTO-1, SYBR-II reveals to be sufficiently sensitive to indicate both DNA or RNA damages as soon as the latter are in contact with hypochlorite even at concentrations of HClO lower than 10 micromol/L. Moreover, SYBR-II offers the opportunity to make quantitative titration of chlorine treated DNA and therefore seems to be the appropriate candidate to control the efficiency of the hypochlorite disinfection process of drinking water samples. |
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Authors:
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M H Phe; M Dossot; J C Block |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Water research Volume: 38 ISSN: 0043-1354 ISO Abbreviation: Water Res. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-07 Completed Date: 2004-12-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0105072 Medline TA: Water Res Country: England |
Other Details:
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Languages: eng Pagination: 3729-37 Citation Subset: IM |
Affiliation:
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Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME) UMR 7564 CNRS, Université Henri Poincaré, Pôle de l'Eau-15, avenue du Charmois, F-54500 Vandoeuvre-lès-Nancy, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA Damage DNA, Bacterial / analysis Environmental Monitoring / methods Fluorescent Dyes / analysis* Sodium Hypochlorite / chemistry Water Microbiology Water Purification* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Fluorescent Dyes; 7681-52-9/Sodium Hypochlorite |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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