Document Detail

Chlorinated-solvent-free gas chromatographic analysis of biomass containing polyhydroxyalkanoates.
MedLine Citation:
PMID:  18355890     Owner:  NLM     Status:  MEDLINE    
An extraction and derivatization method was developed for more environmentally friendly routine quantification of polyhydroxyalkanoates (PHAs) in activated sludge biomass by gas chromatography (GC). This method can be further applied to assess relative changes in biomass carbohydrate levels relating to, for example, glycogen or extracellular polysaccharides. Further, co-extracted principal membrane fatty acids are indicative of relative changes in active biomass. The protocol is attractive for routine assessment because it does not require any chlorinated solvents, which are still almost exclusively used today for PHA analysis by GC. Acidic alcoholysis of dried microbial biomass using 3:1 butanol to concentrated (37%) hydrochloric acid at 100 degrees C for 8 h will hydrolyse and derivatize microbial storage products and membrane lipids. Esters of the hydroxyalkanoates, carbohydrates converted to levulinic acid, and long chain microbial fatty acids are reliably extracted into hexane for gas chromatographic analysis and quantification. Calibration can be achieved with benzoic, 2-hydroxyvaleric, or 2-hydroxycaproic acids as the method reference standards.
Alan Werker; Petter Lind; Simon Bengtsson; Frida Nordström
Publication Detail:
Type:  Journal Article     Date:  2008-02-23
Journal Detail:
Title:  Water research     Volume:  42     ISSN:  0043-1354     ISO Abbreviation:  Water Res.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-12     Completed Date:  2008-09-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0105072     Medline TA:  Water Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  2517-26     Citation Subset:  IM    
AnoxKaldnes Biopolymer AB, Klosterängsvägen 11A, S226 47 Lund, Sweden.
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MeSH Terms
Chlorine / chemistry*
Chromatography, Gas
Esters / chemistry
Flame Ionization
Polyhydroxyalkanoates / analysis*
Solvents / chemistry*
Time Factors
Reg. No./Substance:
0/Esters; 0/Polyhydroxyalkanoates; 0/Solvents; 7782-50-5/Chlorine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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