Document Detail


Chloride cell subtypes in the gill epithelium of Japanese eel Anguilla japonica.
MedLine Citation:
PMID:  10444559     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of the present study was to characterize chloride cell subtypes in the fish gill and to monitor the kinetic change of cell division in the gill epithelia during seawater adaptation. Employing a three-step Percoll gradient method, the gill chloride cells and nonchloride cell population were isolated. The isolated cells were studied using multiparameter flow cytometry, recording the changes in 1) cell size, 2) cellular granularity, and 3) cell autofluorescence. Two chloride cell subtypes were identified in the freshwater eels. Within 2-4 days after entering seawater, new subtypes of transitory chloride cell, with bigger cell size and more intense mitochondria autofluorescence, appeared. After full adaptation, two major seawater chloride cell subtypes were again discerned; their sizes were the largest and their mitochondria autofluorescence was the highest. In the second part of the experiment, cell cycle analysis demonstrated a progressive increase in the percentage of gill cells entering the DNA synthesis phase during seawater adaptation, where a small population of mitotic cells was identified in the nonchloride cell population but not in chloride cells. We hypothesize that the mitotic cells identified are stem cells, which will ultimately differentiate into seawater chloride cells. Our results confirm the existence of heterogeneity of chloride cells. Individual subtypes could be isolated in high purity for further studies to elucidate their respective function in mediating ion transport.
Authors:
C K Wong; D K Chan
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  277     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-23     Completed Date:  1999-09-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R517-22     Citation Subset:  IM    
Affiliation:
Department of Zoology, The University of Hong Kong, Hong Kong. ckcwong@hkbu.edu.hk
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MeSH Terms
Descriptor/Qualifier:
Anguilla / metabolism*
Animals
Cell Size
Chlorides / metabolism*
Coloring Agents
DNA / metabolism
Epithelial Cells / classification,  cytology,  metabolism
Fluorescence
Gills / cytology,  metabolism*
Propidium
Chemical
Reg. No./Substance:
0/Chlorides; 0/Coloring Agents; 36015-30-2/Propidium; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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