Document Detail

Chlorhexidine induces DNA damage in rat peripheral leukocytes and oral mucosal cells.
MedLine Citation:
PMID:  15324357     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Chlorhexidine digluconate is widely used in dental practice for decreasing plaque control, controlling gingivitis and disinfecting root canals. However, the undesirable effects of chlorhexidine digluconate regarding its genotoxicity are conflicting in the literature. Thus, the aim of this study was to investigate the genotoxicity of chlorhexidine digluconate in rat peripheral blood and oral mucosal cells by the single cell gel (comet) assay and micronucleus assay.
METHODS: Thirty male Wistar rats were distributed into three groups: negative control; experimental group orally treated with 0.5 ml of 0.12% chlorhexidine digluconate, twice daily, during 8 days; and positive control, which received 4-nitroquinoline 1-oxide at 0.5 g/l by drinking water.
RESULTS: A statistically significant increase of DNA damage was observed in leukocytes and oral mucosal cells of the chlorhexidine digluconate treated group, as assessed by the comet assay. However, no increase of micronucleated cells was detected in reticulocytes from peripheral blood cells.
CONCLUSIONS: Taken together, the data indicate that chlorhexidine digluconate is able to induce primary DNA damage in leukocytes and in oral mucosal cells, but no chromosome breakage or loss in erythrocytes.
Daniel Araki Ribeiro; Ana Paula Bazo; Carla Adriene da Silva Franchi; Mariângela Esther Alencar Marques; Daisy Maria Favero Salvadori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of periodontal research     Volume:  39     ISSN:  0022-3484     ISO Abbreviation:  J. Periodont. Res.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-08-24     Completed Date:  2004-10-25     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  0055107     Medline TA:  J Periodontal Res     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  358-61     Citation Subset:  D; IM    
Núcleo de Avaliação Toxicogenética e Cancerígena (TOXICAN), Departamento de Patologia, Faculdade de Medicina, UNESP, Botucatu, SP, Brazil.
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MeSH Terms
Anti-Infective Agents, Local / toxicity*
Chlorhexidine / analogs & derivatives*,  toxicity*
Comet Assay
DNA Damage*
Erythrocytes / drug effects
Leukocytes / drug effects*
Micronucleus Tests
Mouth Mucosa / cytology,  drug effects*
Rats, Wistar
Statistics, Nonparametric
Reg. No./Substance:
0/Anti-Infective Agents, Local; 55-56-1/Chlorhexidine; MOR84MUD8E/chlorhexidine gluconate

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