Document Detail

Chloramphenicol residues in chicken liver, kidney and muscle: a comparison among the antibacterial residues monitoring methods of Four Plate Test, ELISA and HPLC.
MedLine Citation:
PMID:  20600543     Owner:  NLM     Status:  MEDLINE    
There are many concerns about safety of food contaminated with antibacterial residues. This study was designed to investigate the occurrence of chloramphenicol (CAP) residue in broiler chickens tissues, namely liver, kidney and muscle. One hundred and sixty broiler chickens carcasses were collected from three provinces of Iran. Four Plate Test (FTP), ELISA and HPLC were used to qualify and quantify the contamination of the samples with CAP. The results of FPT revealed that up to 17.5% of the samples were contaminated with the antibiotic. The ELISA assay showed that out of 28 positive samples in FPT, 22 liver, 21 kidney and 14 muscle samples were positive for CAP. ELISA analyses demonstrated that the minimum and maximum levels of 0.54 and 155.2 ng/g were detected in the kidney and liver, respectively. HPLC analyses confirmed the ELISA findings although the level of contamination was lower than that of ELISA. These data showed that despite the prohibition of CAP application in food animals including poultry, the CAP residue was detectable indicating an illegal use of this antibiotic. Our findings also demonstrated the application of sensitive and more specific analytical assays in screening and quantitation of CAP residues in food products.
H Tajik; H Malekinejad; S M Razavi-Rouhani; M R Pajouhi; R Mahmoudi; A Haghnazari
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-18
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  48     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:    2010 Aug-Sep
Date Detail:
Created Date:  2010-07-26     Completed Date:  2010-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2464-8     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, PO Box 1177, Urmia University, Urmia, Iran.
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MeSH Terms
Anti-Bacterial Agents / analysis*,  pharmacology
Bacteria / drug effects
Biological Assay
Chickens / metabolism*
Chloramphenicol / analysis*,  pharmacology
Chromatography, High Pressure Liquid
Drug Residues / analysis*,  pharmacology
Enzyme-Linked Immunosorbent Assay
Kidney / chemistry*
Liver / chemistry*
Meat / analysis*
Microbial Sensitivity Tests
Muscle, Skeletal / chemistry*
Solid Phase Extraction
Reg. No./Substance:
0/Anti-Bacterial Agents; 56-75-7/Chloramphenicol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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