Document Detail


Chlamydia trachomatis induces remodeling of the actin cytoskeleton during attachment and entry into HeLa cells.
MedLine Citation:
PMID:  12065523     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To elucidate the host cell machinery utilized by Chlamydia trachomatis to invade epithelial cells, we examined the role of the actin cytoskeleton in the internalization of chlamydial elementary bodies (EBs). Treatment of HeLa cells with cytochalasin D markedly inhibited the internalization of C. trachomatis serovar L2 and D EBs. Association of EBs with HeLa cells induced localized actin polymerization at the site of attachment, as visualized by either phalloidin staining of fixed cells or the active recruitment of GFP-actin in viable infected cells. The recruitment of actin to the specific site of attachment was accompanied by dramatic changes in the morphology of cell surface microvilli. Ultrastructural studies revealed a transient microvillar hypertrophy that was dependent upon C. trachomatis attachment, mediated by structural components on the EBs, and cytochalasin D sensitive. In addition, a mutant CHO cell line that does not support entry of C. trachomatis serovar L2 did not display such microvillar hypertrophy following exposure to L2 EBs, which is in contrast to infection with serovar D, to which it is susceptible. We propose that C. trachomatis entry is facilitated by an active actin remodeling process that is induced by the attachment of this pathogen, resulting in distinct microvillar reorganization throughout the cell surface and the formation of a pedestal-like structure at the immediate site of attachment and entry.
Authors:
Reynaldo A Carabeo; Scott S Grieshaber; Elizabeth Fischer; Ted Hackstadt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Infection and immunity     Volume:  70     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-06-14     Completed Date:  2002-07-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3793-803     Citation Subset:  IM    
Affiliation:
Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA.
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MeSH Terms
Descriptor/Qualifier:
1-Phosphatidylinositol 3-Kinase / metabolism
Actins / genetics,  metabolism
Animals
CHO Cells
Chlamydia trachomatis / physiology*
Cricetinae
Cytochalasin D / pharmacology
Cytoskeleton / drug effects,  microbiology,  pathology*
Green Fluorescent Proteins
Hela Cells
Humans
Luminescent Proteins / genetics,  metabolism
Microvilli / pathology
Recombinant Fusion Proteins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Actins; 0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 22144-77-0/Cytochalasin D; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase
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