Document Detail


Chlamydia trachomatis test-of-cure cannot be based on a single highly sensitive laboratory test taken at least 3 weeks after treatment.
MedLine Citation:
PMID:  22470526     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Current test-of-cure practice in patients with Chlamydia trachomatis (Ct) infection is to confirm cure with a single test taken at least 3 weeks after treatment. Effectiveness of single-time-point testing however lacks a scientific evidence basis and the high sensitivity of laboratory assays nowadays in use for this purpose may compromise the clinical significance of their results. Prospectively following 59 treated Ct infections, administering care as usual, the presence of Ct plasmid DNA and rRNA was systematically assessed by multiple time-sequential measurements, i.e. on 18 samples taken per patient during 8 weeks following treatment with a single dose of 1000 mg Azythromycin. A high proportion (42%) of Ct infections tested positive on at least one of the samples taken after 3 weeks. Patients' test results showed substantial inter-individual and intra-individual variation over time and by type of NAAT used. We demonstrated frequent intermittent positive patterns in Ct test results over time, and strongly argue against current test-of-cure practice.
Authors:
Nicole H T M Dukers-Muijrers; Servaas A Morré; Arjen Speksnijder; Marianne A B van der Sande; Christian J P A Hoebe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-03-28
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-03     Completed Date:  2012-08-03     Revised Date:  2013-05-20    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e34108     Citation Subset:  IM    
Affiliation:
Department of Sexual Health, Infectious Diseases and Environmental Health, Geleen, South Limburg Public Health Service, South Limburg, The Netherlands. nicole.dukers@ggdzl.nl
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / therapeutic use*
Azithromycin / therapeutic use*
Chlamydia Infections / diagnosis*,  drug therapy*,  microbiology
Chlamydia trachomatis / genetics*,  isolation & purification
DNA, Bacterial / analysis
Female
Humans
Male
RNA, Ribosomal / analysis
Time Factors
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/DNA, Bacterial; 0/RNA, Ribosomal; 83905-01-5/Azithromycin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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