Document Detail

Chitosan prevents the development of AOM-induced aberrant crypt foci in mice and suppressed the proliferation of AGS cells by inhibiting DNA synthesis.
MedLine Citation:
PMID:  17226752     Owner:  NLM     Status:  MEDLINE    
We study the effect of fungal-derived chitosan on the development of chemical-induced colonic precancerous lesions in ICR mice and delineate its possible molecular mechanisms. In the 2 weeks preventive experiments, mice fed with a diet containing high molecular weight chitosan (HMWC) had significant fewer aberrant crypt foci formation than those fed with control diet. As the treatment extended to 6 weeks, both low molecular weight chitosan (LMWC)- and HMWC-fed mice contained less aberrant crypt foci when compared to control. However, such effect was not observed in mice in the 6 weeks therapeutic experiments. The anti-tumorigenesis effect of water-soluble chitosan oligomer (WSCO) was tested on four cancer cell lines. WSCO significantly suppressed AGS and to a less extent, COLO 205 cells proliferation. Flow cytometry analysis of cell cycle distribution indicated that the percentage of S phase reduced significantly in AGS cells treated with WSCO together with a decrease in DNA synthesis rate in BrdU incorporation assay. WSCO treatment also upregulated cell cycle-related genes p21/Cip and p27/Kip, whereas downregulated that of PCNA.
Shyr-Yi Lin; Hing-Yuen Chan; Fung-Hsiu Shen; Mei-Huei Chen; Ying-Jan Wang; Chung-Keung Yu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  100     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-27     Completed Date:  2007-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1573-80     Citation Subset:  IM    
Department of Internal Medicine, School of Medicine, Taipei Medical University and Hospital, Taipei, Taiwan.
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MeSH Terms
Azoxymethane / toxicity*
Blotting, Western
Carcinogens / toxicity
Cell Cycle / drug effects
Cell Cycle Proteins / metabolism
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
Chitosan / administration & dosage,  chemistry,  pharmacology*
Colonic Neoplasms / chemically induced,  pathology,  prevention & control*
DNA Replication / drug effects*
Flow Cytometry
Jurkat Cells
Mice, Inbred ICR
Molecular Weight
Precancerous Conditions / chemically induced,  pathology,  prevention & control*
Stomach Neoplasms / metabolism,  pathology
Time Factors
Urinary Bladder Neoplasms / metabolism,  pathology
Reg. No./Substance:
0/Carcinogens; 0/Cell Cycle Proteins; 0/water soluble chitin; 25843-45-2/Azoxymethane; 9012-76-4/Chitosan

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