| Chitosan prevents the development of AOM-induced aberrant crypt foci in mice and suppressed the proliferation of AGS cells by inhibiting DNA synthesis. | |
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MedLine Citation:
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PMID: 17226752 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We study the effect of fungal-derived chitosan on the development of chemical-induced colonic precancerous lesions in ICR mice and delineate its possible molecular mechanisms. In the 2 weeks preventive experiments, mice fed with a diet containing high molecular weight chitosan (HMWC) had significant fewer aberrant crypt foci formation than those fed with control diet. As the treatment extended to 6 weeks, both low molecular weight chitosan (LMWC)- and HMWC-fed mice contained less aberrant crypt foci when compared to control. However, such effect was not observed in mice in the 6 weeks therapeutic experiments. The anti-tumorigenesis effect of water-soluble chitosan oligomer (WSCO) was tested on four cancer cell lines. WSCO significantly suppressed AGS and to a less extent, COLO 205 cells proliferation. Flow cytometry analysis of cell cycle distribution indicated that the percentage of S phase reduced significantly in AGS cells treated with WSCO together with a decrease in DNA synthesis rate in BrdU incorporation assay. WSCO treatment also upregulated cell cycle-related genes p21/Cip and p27/Kip, whereas downregulated that of PCNA. |
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Authors:
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Shyr-Yi Lin; Hing-Yuen Chan; Fung-Hsiu Shen; Mei-Huei Chen; Ying-Jan Wang; Chung-Keung Yu |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 100 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-27 Completed Date: 2007-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1573-80 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, School of Medicine, Taipei Medical University and Hospital, Taipei, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Azoxymethane / toxicity* Blotting, Western Carcinogens / toxicity Cell Cycle / drug effects Cell Cycle Proteins / metabolism Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Chitosan / administration & dosage, chemistry, pharmacology* Colonic Neoplasms / chemically induced, pathology, prevention & control* DNA Replication / drug effects* Flow Cytometry Humans Jurkat Cells Male Mice Mice, Inbred ICR Molecular Weight Precancerous Conditions / chemically induced, pathology, prevention & control* Stomach Neoplasms / metabolism, pathology Time Factors Urinary Bladder Neoplasms / metabolism, pathology |
| Chemical | |
Reg. No./Substance:
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0/Carcinogens; 0/Cell Cycle Proteins; 0/water soluble chitin; 25843-45-2/Azoxymethane; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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