Document Detail

Chitosan permeabilizes the plasma membrane and kills cells of Neurospora crassa in an energy dependent manner.
MedLine Citation:
PMID:  19389478     Owner:  NLM     Status:  MEDLINE    
Chitosan has been reported to inhibit spore germination and mycelial growth in plant pathogens, but its mode of antifungal action is poorly understood. Following chitosan treatment, we characterized plasma membrane permeabilization, and cell death and lysis in the experimental model, Neurospora crassa. Rhodamine-labeled chitosan was used to show that chitosan is internalized by fungal cells. Cell viability stains and the calcium reporter, aequorin, were used to monitor plasma membrane permeabilization and cell death. Chitosan permeabilization of the fungal plasma membrane and its uptake into fungal cells was found to be energy dependent but not to involve endocytosis. Different cell types (conidia, germ tubes and vegetative hyphae) exhibited differential sensitivity to chitosan with ungerminated conidia being the most sensitive.
J Palma-Guerrero; I-C Huang; H-B Jansson; J Salinas; L V Lopez-Llorca; N D Read
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-21
Journal Detail:
Title:  Fungal genetics and biology : FG & B     Volume:  46     ISSN:  1096-0937     ISO Abbreviation:  Fungal Genet. Biol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-06-17     Completed Date:  2009-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607601     Medline TA:  Fungal Genet Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  585-94     Citation Subset:  IM    
Fungal Cell Biology Group, Institute of Cell Biology, University of Edinburgh, Rutherford Building, Edinburgh, UK.
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MeSH Terms
Antifungal Agents / pharmacology*
Cell Membrane / drug effects*
Cell Membrane Permeability / drug effects*
Chitosan / pharmacology*
Microbial Viability*
Neurospora crassa / drug effects*,  physiology
Grant Support
BB/E010741/1//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Antifungal Agents; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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