Document Detail

Chitosan: a new solution for removal of smear layer after root canal instrumentation.
MedLine Citation:
PMID:  22970844     Owner:  NLM     Status:  Publisher    
AIM: To evaluate, by scanning electron microscopy (SEM), the efficacy of smear layer removal using chitosan compared with different chelating agents, and to quantify, by atomic absorption spectrophotometry with flame (AASF), the concentration of calcium ions in these solutions after irrigation. METHODOLOGY: The root canals of twenty-five canines were prepared using a crown-down technique and irrigated with 1% sodium hypochlorite. The teeth were randomly divided into groups (n = 5), according to the type of final irrigation: 15% EDTA, 0.2% chitosan, 10% citric acid, 1% acetic acid and control (without final irrigation). The total volume of each chelating solution was collected from the canals and analysed by AASF for quantification of calcium ions in the solutions. Then, the roots were split longitudinally and examined by SEM for evaluation of smear layer removal in the middle and apical thirds. Cleaning scores were attributed and analysed statistically using the Kruskal-Wallis and Dunn tests. The AASF data were analysed by one-way anova and Tukey-Kramer test. A significant level of α = 0.05 was adopted. RESULTS: 15% EDTA, 0.2% chitosan and 10% citric acid had similar smear layer removal capacity with a significant difference (P < 0.05) from 1% acetic acid and the control group. There was no significant difference (P > 0.05) between the smear layer remaining in the middle and apical thirds. The highest calcium ion concentration was observed with 15% EDTA (121.80 ± 5.13) and 0.2% chitosan (104.13 ± 19.23), with no significant difference. The lowest calcium ion concentration was obtained with 1% acetic acid (25.62 ± 7.68), whilst 10% citric acid (70.38 ± 11.15) had intermediate results, differing significantly from the other solutions (P < 0.01). CONCLUSIONS: 15% EDTA, 0.2% chitosan and 10% citric acid effectively removed smear layer from the middle and apical thirds of the root canal. 15% EDTA and 0.2% chitosan were associated with the greatest effect on root dentine demineralization, followed by 10% citric acid and 1% acetic acid.
P V Silva; D F C Guedes; F V Nakadi; J D Pécora; A M Cruz-Filho
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-26
Journal Detail:
Title:  International endodontic journal     Volume:  -     ISSN:  1365-2591     ISO Abbreviation:  Int Endod J     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-9-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8004996     Medline TA:  Int Endod J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 International Endodontic Journal.
Department of Restorative Dentistry, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
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