| Chitosan-mediated stimulation of macrophage function. | |
| | |
MedLine Citation:
|
PMID: 7703317 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
According to the modern definition of biocompatibility, a biocompatible material need not be inert but be bioactive. A benign reactivity implies that the reactivity has to be appropriate for the intended use. Chitosan, a non-acetylated or partially deacetylated chitin (a linear homopolymer of beta (1-4)-linked N-acetylglucosamine) has been proposed as a biomaterial because of its apparent satisfactory biocompatibility. The present investigation demonstrates that chitosan has an in vitro stimulatory effect on both macrophage nitric oxide (NO) production and chemotaxis. The macrophage NO secretion is attributed to the N-acetylglucosamine unit of the chitosan molecule rather than to the glucosamine residue (28 and 15 microM NO respectively). Moreover, the immune stimulatory effect of chitosan was very specific since other glycosaminoglycans, such as N-acetyl-D-mannosamine and N-acetyl-D-galactosamine, had no effects on NO production (5 and 8 respectively). In vivo experiments strengthen this hypothesis. Transmission electron microscopy analysis identifies the presence of many leucocytes in the specimens after 14 d post-implantation, showing poor healing processes (i.e. fibroblast proliferation and collagen deposition) that characterize the tissue repair at this time in our animal model. |
| | |
Authors:
|
G Peluso; O Petillo; M Ranieri; M Santin; L Ambrosio; D Calabró; B Avallone; G Balsamo |
Related Documents
:
|
1650307 - Inhibition of alveolar macrophages by pentoxifylline. 8031127 - Macrophage recognition of saccharide chains on the erythrocytes damaged by iron-catalyz... 18604417 - Lysozyme plays a dual role against the dimorphic fungus paracoccidioides brasiliensis. 7503067 - In vitro effects of bicarbonate- versus lactate-buffered continuous ambulatory peritone... 3181817 - Intravascular coagulation phenomena associated with prevalent fall in fibrinogen and pl... 17848327 - Phase i/ii prospective trial of autologous platelet tissue graft in gynecologic surgery. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biomaterials Volume: 15 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 1994 Dec |
Date Detail:
|
Created Date: 1995-05-09 Completed Date: 1995-05-09 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 1215-20 Citation Subset: IM |
Affiliation:
|
Institute of Protein Biochemistry and Enzymology, CNB, Naples, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylglucosamine
/
pharmacology Animals Biocompatible Materials / pharmacology* Chemotactic Factors / pharmacology Chemotaxis / drug effects Chitin / analogs & derivatives*, pharmacology Chitosan Concanavalin A / pharmacology Glycosaminoglycans / pharmacology Hemostatics / pharmacology Leukocytes / cytology, drug effects Macrophage Activation / drug effects, physiology Macrophages, Peritoneal / drug effects*, metabolism, physiology* Male Microscopy, Electron Nitric Oxide / biosynthesis Nitrites / metabolism Rats Rats, Wistar Stimulation, Chemical |
| Chemical | |
Reg. No./Substance:
|
0/Biocompatible Materials; 0/Chemotactic Factors; 0/Glycosaminoglycans; 0/Hemostatics; 0/Nitrites; 10102-43-9/Nitric Oxide; 11028-71-0/Concanavalin A; 1398-61-4/Chitin; 7512-17-6/Acetylglucosamine; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Biodegradation of PLA/GA polymers: increasing complexity.
Next Document: Photopolymerization of dental composite matrices.