Document Detail

Chitinous components of the cell wall of Fusarium oxysporum.
MedLine Citation:
PMID:  1282049     Owner:  NLM     Status:  MEDLINE    
The cell wall of Fusarium oxysporum f. sp. lycopersici was digested with chitinase to analyze the structure of its chitinous components. In spite of a similar acetylation degree of the cell wall components to that of 25-35% acetylated chitosan, only N-acetylglucosamine disaccharide [(GlcNAc)2] was obtained from chitinase hydrolyzate of the fungal cell wall by CM-Sephadex C-25 column chromatography, while (GlcNAc)2 and several types of deacetylated chitooligosaccharides were separated from that of 25-35% acetylated chitosan. The results indicate that N-acetylglucosamine residues in the polysaccharide chains of the fungal cell wall are most likely condensed into some region, while acetylated residues are more scattered in 25-35% acetylated chitosan.
T Fukamizo; T Ohkawa; K Sonoda; H Toyoda; T Nishiguchi; S Ouchi; S Goto
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  56     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1993-02-02     Completed Date:  1993-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1632-6     Citation Subset:  B    
Laboratory of Biophysical Chemistry, Faculty of Agriculture, Kinki University, Nara, Japan.
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MeSH Terms
Carbohydrate Sequence
Carbohydrates / analysis
Cell Wall / chemistry
Chitin / analogs & derivatives,  analysis*
Chitinase / metabolism
Chromatography / methods
Fusarium / chemistry*,  ultrastructure
Molecular Sequence Data
Spectrophotometry, Infrared
Reg. No./Substance:
0/Carbohydrates; 1398-61-4/Chitin; 9004-54-0/Dextrans; 9012-76-4/Chitosan; 9014-76-0/sephadex; EC

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