Document Detail

Chitin-binding proteins of Artemia diapause cysts participate in formation of the embryonic cuticle layer of cyst shells.
MedLine Citation:
PMID:  23013449     Owner:  NLM     Status:  Publisher    
The brine shrimp, Artemia, reproduces either ovoviviparously, producing free-swimming nauplii, or oviparously, producing encysted embryos (diapause cysts) able to cope with harsh and complex habitats. When the cysts enter diapause they are encased in a complex external shell that protects them from certain extreme environments. The genomic comparison of oviparous and ovoviviparous ovisacs was described previously. We isolated three significantly up-regulated genes in oviparous oocytes and identified them as Arp-CBP genes (Artemia parthenogenetica chitin-binding protein gene). Quantitative RT-PCR indicated that the expression of Arp-CBP genes gradually increases during diapause cyst formation and significant mRNA accumulation occurs during the ovisac stage of oviparous development. Moreover, in situ hybridization results demonstrated that Arp-CBP mRNAs are expressed in the embryo. Interestingly, the results of immune electron microscopy showed that all three Arp-CBPs are distributed throughout the cellular embryonic cuticle layer of the cyst shell. Furthermore, knock-down of Arp-CBP by RNAi resulted in marked changes in the composition of the embryonic cuticular layer. The fibrous layer of the cyst shell adopted a loose conformation and the inner cuticular membrane and outer cuticular membrane exhibited marked irregularities when Arp-CBP expression was suppressed. Finally, an in vitro recombinant protein binding assay showed that all three Arp-CBPs have carbohydrate-binding activities. These findings provide significant insight into the mechanisms by which the embryonic cuticle layer of Artemia cyst shell is formed, and demonstrate that Arp-CBPs are involved in construction of the fibrous lattice and are required for formation of the embryonic cuticle layer of the cyst shell.
Wen-Ming Ma; Hua-Wei Li; Zhong-Min Dai; Jin-Shu Yang; Fan Yang; Wei-Jun Yang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-26
Journal Detail:
Title:  The Biochemical journal     Volume:  -     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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