| Chitin-binding proteins of Artemia diapause cysts participate in formation of the embryonic cuticle layer of cyst shells. | |
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MedLine Citation:
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PMID: 23013449 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The brine shrimp, Artemia, reproduces either ovoviviparously, producing free-swimming nauplii, or oviparously, producing encysted embryos (diapause cysts) able to cope with harsh and complex habitats. When the cysts enter diapause they are encased in a complex external shell that protects them from certain extreme environments. The genomic comparison of oviparous and ovoviviparous ovisacs was described previously. We isolated three significantly up-regulated genes in oviparous oocytes and identified them as Arp-CBP genes (Artemia parthenogenetica chitin-binding protein gene). Quantitative RT-PCR indicated that the expression of Arp-CBP genes gradually increases during diapause cyst formation and significant mRNA accumulation occurs during the ovisac stage of oviparous development. Moreover, in situ hybridization results demonstrated that Arp-CBP mRNAs are expressed in the embryo. Interestingly, the results of immune electron microscopy showed that all three Arp-CBPs are distributed throughout the cellular embryonic cuticle layer of the cyst shell. Furthermore, knock-down of Arp-CBP by RNAi resulted in marked changes in the composition of the embryonic cuticular layer. The fibrous layer of the cyst shell adopted a loose conformation and the inner cuticular membrane and outer cuticular membrane exhibited marked irregularities when Arp-CBP expression was suppressed. Finally, an in vitro recombinant protein binding assay showed that all three Arp-CBPs have carbohydrate-binding activities. These findings provide significant insight into the mechanisms by which the embryonic cuticle layer of Artemia cyst shell is formed, and demonstrate that Arp-CBPs are involved in construction of the fibrous lattice and are required for formation of the embryonic cuticle layer of the cyst shell. |
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Authors:
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Wen-Ming Ma; Hua-Wei Li; Zhong-Min Dai; Jin-Shu Yang; Fan Yang; Wei-Jun Yang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-26 |
Journal Detail:
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Title: The Biochemical journal Volume: - ISSN: 1470-8728 ISO Abbreviation: Biochem. J. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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