Document Detail

Chiral separation of NBD-amino acids by ligand-exchange micro-channel electrophoresis.
MedLine Citation:
PMID:  15675519     Owner:  NLM     Status:  MEDLINE    
The chiral separation of amino acid derivatives by ligand-exchange electrophoresis in a microchannel chip was performed for the first time. A Cu(II) complex with L-prolinamide was used as a chiral selector. The migration behaviors of eleven NBD-DL-amino acids were investigated by ligand-exchange capillary electrophoresis (LE-CE). The enantiomer of five NBD-amino acids (Ser, Thr, Val, Phe and His) could be separated by LE-CE using a 20 mM ammonium acetate buffer (pH 9.0) containing 10 mM copper acetate, 20 mM L-prolinamide and 1 mM SDS. NBD-His was eluted in the order D-form and L-form, while the elution order of another enantiomers was L-form and D-form. Under this condition, the enantioseparation of these five NBD-amino acids by ligand-exchange microchip electrophoresis (LE-ME) was investigated using a glass microchip. The enantioseparation of NBD-Ser, -Thr and -His could be successfully accomplished by LE-ME. LE-ME was superior to LE-CE in terms of the short migration time and a good enantiomeric separation.
Hizuru Nakajima; Katsuhiko Kawata; Hui Shen; Tatsuro Nakagama; Katsumi Uchiyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  21     ISSN:  0910-6340     ISO Abbreviation:  Anal Sci     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-28     Completed Date:  2005-03-10     Revised Date:  2005-10-11    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  67-71     Citation Subset:  IM    
Department of Applied Chemistry, Graduate School of Engineering, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
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MeSH Terms
4-Chloro-7-nitrobenzofurazan / analogs & derivatives*
Amino Acids / isolation & purification*
Electrophoresis, Microchip*
Proline / analogs & derivatives*
Reg. No./Substance:
0/Amino Acids; 0/Ligands; 10199-89-0/4-Chloro-7-nitrobenzofurazan; 147-85-3/Proline; 29270-56-2/7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; 7440-50-8/Copper; 7531-52-4/prolinamide

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