Document Detail

Chiral analysis of amino acids using electrochemical composite bienzyme biosensors.
MedLine Citation:
PMID:  11700983     Owner:  NLM     Status:  MEDLINE    
The construction and performance of bienzyme amperometric composite biosensors for the selective determination of l- or d-amino acids is reported. D- or L-Amino acid oxidase, horseradish peroxidase, and the mediator ferrocene were coimmobilized by simple physical inclusion into the bulk of a graphite-70% Teflon electrode matrix. Working conditions including amino acid oxidase loading and pH were optimized. Studies on the repeatability of the amperometric response obtained at +0.00 V, with and without regeneration of the electrode surface by polishing, on the useful lifetime of one single biosensor and on the reproducibility in the fabrication of different biosensors illustrate the robustness of the bioelectrodes design. Calibration plots by both amperometry in stirred solutions and flow injection with amperometric detection were obtained for L-arginine, L-phenylalanine, L-leucine, L-methionine, L-tryptophan, D-leucine, D-methionine, D-serine, and D-valine. Differences in sensitivity were discussed in terms of the hydrophobicity of the substrate and of the electrode surface. The bienzyme composite electrode was applied to the determination of L- and D-amino acids in racemic samples, as well as to the estimation of the L-amino acids content in muscatel grapes.
R Domínguez; B Serra; A J Reviejo; J M Pingarrón
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  298     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-09     Completed Date:  2002-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  275-82     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Analytical Chemistry, Complutense University of Madrid, Madrid, 28040, Spain.
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MeSH Terms
Amino Acid Oxidoreductases / chemistry
Amino Acids / analysis*
Biosensing Techniques / methods*
D-Amino-Acid Oxidase / chemistry
Electrochemistry / methods*
Ferrous Compounds / chemistry
Graphite / chemistry
Horseradish Peroxidase / chemistry
L-Amino Acid Oxidase
Polytetrafluoroethylene / chemistry
Vitis / metabolism
Reg. No./Substance:
0/Amino Acids; 0/Ferrous Compounds; 102-54-5/ferrocene; 7782-42-5/Graphite; 9002-84-0/Polytetrafluoroethylene; EC 1.11.1.-/Horseradish Peroxidase; EC 1.4.-/Amino Acid Oxidoreductases; EC Acid Oxidase; EC Oxidase

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