Document Detail


Chip-calorimetry provides real time insights into the inactivation of biofilms by predatory bacteria.
MedLine Citation:
PMID:  22509741     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Control or removal of undesired biofilms has frequently been found to be quite difficult. In addition to biocidal or antibiotic chemicals or materials designed to prevent biofouling, biological control agents appear to be promising. Reports of bacterial predators eradicating biofilms or eliminating pathogens motivate a more systematic screening of biofilm-eliminating bacterial predators. Unfortunately, the analysis of the eradication process is demanding. In the present study, chip-calorimetry was applied to monitor the elimination of Pseudomonas sp. biofilms by Bdellovibrio bacteriovorus. The method uses metabolic heat as a real-time parameter for biofilm activity. The method is non-invasive, fast and convenient due to real-time data acquisition. In addition, heat-production data can reveal information about the energetics of the predator-prey interaction. The calorimetric results were validated by confocal laser scanning microscopy. The approach described may be useful for the screening of biofilm susceptibility to different predators.
Authors:
F Buchholz; J Lerchner; F Mariana; U Kuhlicke; T R Neu; H Harms; T Maskow
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biofouling     Volume:  28     ISSN:  1029-2454     ISO Abbreviation:  Biofouling     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-04-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200331     Medline TA:  Biofouling     Country:  England    
Other Details:
Languages:  eng     Pagination:  351-62     Citation Subset:  IM    
Affiliation:
a Department of Environmental Microbiology , UFZ-Helmholtz Centre for Environmental Research , Leipzig , Germany.
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