Document Detail

On-chip dielectrophoretic coassembly of live cells and particles into responsive biomaterials.
MedLine Citation:
PMID:  19957941     Owner:  NLM     Status:  MEDLINE    
We report how live cells and functionalized colloidal particles can be coassembled into a variety of freely suspended bioactive structures using dielectrophoresis on a chip. Alternating electric fields were applied to dilute suspensions of yeast (S. cerevisiae) and NIH/3T3 mouse fibroblast cells to yield 1D chains and 2D arrays. The effects of voltage, frequency, pH, electrolyte concentration, cell concentration, and particle size on the assembly process were investigated in detail. Numerical simulations of the field intensity and energy allow the capture of the dynamics of cell-cell and cell-particle assembly. The simulation results illustrate that the electric field draws the functionalized synthetic particles between the cells and enables the formation of permanent chains and monolayer membranes composed of alternating cells and particles. The cell structures were bound into permanent structures by different types of functionalized synthetic particles and ligands that attached to the cells through biospecific or electrostatic interactions. The technique allowed the fabrication of magnetically responsive biomaterials that could be manipulated and transported into and out of the microchambers where they were formed.
Shalini Gupta; Rossitza G Alargova; Peter K Kilpatrick; Orlin D Velev
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  26     ISSN:  1520-5827     ISO Abbreviation:  Langmuir     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-23     Completed Date:  2010-05-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3441-52     Citation Subset:  IM    
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905, USA.
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MeSH Terms
Cell Adhesion
Cell Membrane / metabolism
Cell Survival
Electrolytes / chemistry
Electrophoresis / instrumentation*
Fibroblasts / cytology
Hydrogen-Ion Concentration
Microchip Analytical Procedures*
NIH 3T3 Cells
Saccharomyces cerevisiae / cytology
Static Electricity
Reg. No./Substance:
0/Colloids; 0/Electrolytes; 0/Latex

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