Document Detail

Chinese hamster ovary cells resistant to borrelidin overproduce threonyl-tRNA synthetase.
MedLine Citation:
PMID:  6746646     Owner:  NLM     Status:  MEDLINE    
Chinese hamster ovary cell lines that are 1000-fold more resistant to the threonyl-tRNA synthetase inhibitor borrelidin than the sensitive parental cells were isolated after stepwise selection for growth in increasing concentrations of the drug. These cells show a 10-20-fold increase in threonyl-tRNA synthetase activity. Quantitation of the amount of threonyl-tRNA synthetase protein by immunological techniques indicated a 60-100-fold increase compared to sensitive cells. No significant changes in the Km for substrates, inhibition by borrelidin or thermal stability were found for the threonyl-tRNA synthetase of resistant cells. These data suggest that the resistant cell lines may have amplified the gene encoding threonyl-tRNA synthetase, but no evidence of homogeneously staining regions or double minute chromosomes was found. The resistant cell lines should prove useful for the study of the regulation of threonyl-tRNA synthetase.
S C Gerken; S M Arfin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  259     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1984 Jul 
Date Detail:
Created Date:  1984-08-27     Completed Date:  1984-08-27     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9202-6     Citation Subset:  IM    
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MeSH Terms
Amino Acyl-tRNA Synthetases / biosynthesis*
Anti-Bacterial Agents / toxicity*
Cell Line
Drug Resistance
Fatty Alcohols / toxicity
Threonine-tRNA Ligase / antagonists & inhibitors,  biosynthesis*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Fatty Alcohols; 7184-60-3/borrelidin; EC 6.1.1.-/Amino Acyl-tRNA Synthetases; EC Ligase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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