| Chinese hamster ovary cells resistant to borrelidin overproduce threonyl-tRNA synthetase. | |
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MedLine Citation:
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PMID: 6746646 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chinese hamster ovary cell lines that are 1000-fold more resistant to the threonyl-tRNA synthetase inhibitor borrelidin than the sensitive parental cells were isolated after stepwise selection for growth in increasing concentrations of the drug. These cells show a 10-20-fold increase in threonyl-tRNA synthetase activity. Quantitation of the amount of threonyl-tRNA synthetase protein by immunological techniques indicated a 60-100-fold increase compared to sensitive cells. No significant changes in the Km for substrates, inhibition by borrelidin or thermal stability were found for the threonyl-tRNA synthetase of resistant cells. These data suggest that the resistant cell lines may have amplified the gene encoding threonyl-tRNA synthetase, but no evidence of homogeneously staining regions or double minute chromosomes was found. The resistant cell lines should prove useful for the study of the regulation of threonyl-tRNA synthetase. |
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Authors:
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S C Gerken; S M Arfin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 259 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1984 Jul |
Date Detail:
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Created Date: 1984-08-27 Completed Date: 1984-08-27 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9202-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acyl-tRNA Synthetases
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biosynthesis* Animals Anti-Bacterial Agents / toxicity* Cell Line Cricetinae Cricetulus Drug Resistance Fatty Alcohols / toxicity Female Kinetics Ovary Threonine-tRNA Ligase / antagonists & inhibitors, biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Fatty Alcohols; 7184-60-3/borrelidin; EC 6.1.1.-/Amino Acyl-tRNA Synthetases; EC 6.1.1.3/Threonine-tRNA Ligase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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