Document Detail

A Chinese hamster ovary cell mutant resistant to phosphatidylserine is defective in transbilayer movement of cell surface phosphatidylserine.
MedLine Citation:
PMID:  8912728     Owner:  NLM     Status:  MEDLINE    
A mammalian plasma membrane protein(s) which catalyzes ATP-dependent transbilayer movement (flip-flop) of phosphatidylserine (PS) has been suggested to be involved in the formation and maintenance of membrane lipid asymmetry. Flip-flop of PS in the cell surface of nucleated cells was first described by O. C. Martin and R. E. Pagano (1987, J. Biol. Chem. 262, 5890-5898). It has been suggested that flip-flop is involved in the internalization of exogenous PS in cultured cells. In the present study we report that incubation with an excess amount of PS is cytotoxic to Chinese hamster ovary (CHO) cells, while the same amount of phosphatidylcholine gives no effect. This effect allowed us to obtain PS-resistant cells among mutagenized CHO cells. Endocytosis-independent internalization of exogenous fluorescent PS analog was defective in 40% of the PS-resistant mutants. One of the mutants, PSR (phosphatidylserine resistant) 406 was further characterized. Unlike wild-type CHO cells, this mutant did not transport fluorescent PS significantly at 15 degrees C. Fluorescent PS was not metabolized at 15 degrees C in either wild-type or mutant cells. These results suggest that transbilayer movement of cell surface PS is defective in PS-resistant cells.
T A Endo; T Kobayashi; K Ohki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  228     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1996-12-16     Completed Date:  1996-12-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  341-6     Citation Subset:  IM    
Department of Physics, Tohoku University, Sendai, Japan.
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MeSH Terms
CHO Cells
Cell Survival / drug effects
Drug Resistance* / genetics
Fluorescent Dyes
Lipid Bilayers*
Membrane Lipids / metabolism*
Phosphatidylserines / metabolism*,  pharmacology*
Reg. No./Substance:
0/Fluorescent Dyes; 0/Lipid Bilayers; 0/Membrane Lipids; 0/Phosphatidylserines

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