| Chinese hamster ORC subunits dynamically associate with chromatin throughout the cell-cycle. | |
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MedLine Citation:
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PMID: 15950218 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In yeast, the Origin Recognition Complex (ORC) is bound to replication origins throughout the cell-cycle, but in animal cells, there are conflicting data as to whether and when ORC is removed from chromatin. We find ORC1, 2 and ORC4 to be metabolically stable proteins that co-fractionate with chromatin throughout the cell-cycle in Chinese hamster fibroblasts. Since cellular extraction methods cannot directly examine the chromatin binding properties of proteins in vivo, we examined ORC:chromatin interactions in living cells. Fluorescence loss in photobleaching (FLIP) studies revealed ORC1 and ORC4 to be highly dynamic proteins during the cell-cycle with exchange kinetics similar to other regulatory chromatin proteins. In vivo interaction with chromatin was not significantly altered throughout the cell-cycle, including S-phase. These data support a model in which ORC subunits dynamically interact with chromatin throughout the cell-cycle. |
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Authors:
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Adrian J McNairn; Yukiko Okuno; Tom Misteli; David M Gilbert |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental cell research Volume: 308 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-01 Completed Date: 2005-09-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 345-56 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, S.U.N.Y. Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells Cell Cycle / physiology* Cell Nucleus / metabolism* Chromatin / metabolism* Cricetinae DNA Replication / physiology DNA-Binding Proteins / metabolism* Origin Recognition Complex Photobleaching Protein Subunits / metabolism* S Phase / physiology |
| Grant Support | |
ID/Acronym/Agency:
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GM-57233-01/GM/NIGMS NIH HHS; Z01 BC010309-07/BC/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/DNA-Binding Proteins; 0/Origin Recognition Complex; 0/Protein Subunits |
| Comments/Corrections | |
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