Document Detail


Chimeric protein A/G conjugate for detection of anti-Toxoplasma gondii immunoglobulin G in multiple animal species.
MedLine Citation:
PMID:  22529128     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Serological testing for toxoplasmosis diagnosis remains the method of choice in human medicine due to the accessibility of the requisite sample, the difficulty in predicting the parasite's location in the host for direct detection, and the availability of established commercial methods. In veterinary medicine, although the first 2 conditions are unchanged, there is a need for commercially produced test methods that are validated for Toxoplasma gondii detection across the range of animal species that can serve as intermediate hosts. The development of such a serological method for animals would allow the diagnosis of toxoplasmosis in individual animals and a higher throughput method for population-level toxoplasmosis surveys. The incorporation of a non-species-specific chimeric protein A/G conjugate into an anti-Toxoplasma immunoglobulin G enzyme-linked immunosorbent assay is described. Serum from potential intermediate hosts was reevaluated using this method and compared with earlier testing using an established agglutination procedure. Very good agreement between the 2 tests was noted (κ = 0.81), establishing the method as a useful option for veterinary diagnostic testing.
Authors:
John J Schaefer; Holly A White; Stephanie L Schaaf; Hussni O Mohammed; Susan E Wade
Related Documents :
22428358 - Adapting the everyday discrimination scale to medical settings: reliability and validit...
22675968 - Comparison of diclofenac sodium with indomethacin suppositories for mediolateral episio...
22506168 - The test-retest reliability of supraspinatus cross-sectional area measurement by sonogr...
22915608 - Standardized methods and quality control limits for agar and broth microdilution suscep...
20046938 - Bis, bas, and response conflict: testing predictions of the revised reinforcement sensi...
3770658 - Hormonal regulation of male reproductive behavior in the lizard anolis sagrei: a test o...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc     Volume:  24     ISSN:  1943-4936     ISO Abbreviation:  J. Vet. Diagn. Invest.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9011490     Medline TA:  J Vet Diagn Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  572-5     Citation Subset:  IM    
Affiliation:
1Susan E. Wade, Animal Health Diagnostic Center, Farrier Road, Cornell University, Ithaca, NY 14853. sew9@cornell.edu.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A rapid mismatch polymerase chain reaction assay to detect carriers of complex vertebral malformatio...
Next Document:  Detection of six novel papillomavirus sequences within canine pigmented plaques.