Document Detail


Chimeric monoclonal antibodies to hypervariable region 1 of hepatitis C virus.
MedLine Citation:
PMID:  15914849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two chimeric monoclonal antibodies (cAbs), 2P24 and 15H4, to hypervariable region 1 (HVR1) of hepatitis C virus (HCV) were constructed by grafting the variable regions of murine monoclonal antibodies (mAbs) 2P24 and 15H4 to a human IgG1 kappa constant region. Two cAb-producing cell lines were adapted to serum-free media. Both cAb 2P24 and cAb 15H4 cell lines produced 3-5 microg antibodies ml(-1) after 3-5 days culture. cAbs retained binding characteristics similar to those observed in the original mAbs. There was no clear difference in affinity between binding of cAbs and mAbs to seven HVR1 peptides. Mixtures of biotinylated cAbs or mAbs reacted with 32 (86 %) and 31 (84 %) of 37 HVR1 peptides, respectively, but not with non-HVR1 control peptides. HCV from 16 out of 18 (89 %) random HCV-containing plasmas was captured by the mixture of biotinylated cAbs. The capture from IgG-depleted plasmas suggested that cAbs captured mainly free rather than complexed HCV, irrespective of genotype. A mixture of the two cAbs inhibited HCV binding to Molt-4 cells in a dose-dependent manner. These cAbs may be useful for prevention of nosocomial HCV infection and passive immunization to prevent HCV reinfection after liver transplantation.
Authors:
Chengyao Li; Jean-Pierre Allain
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general virology     Volume:  86     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-25     Completed Date:  2005-07-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1709-16     Citation Subset:  IM    
Affiliation:
National Blood Service, Division of Transfusion Medicine, East Anglia Blood Centre, Long Road, Cambridge CB2 2PT, UK.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal / biosynthesis,  immunology
Antibody Specificity
Hepacivirus / immunology*,  isolation & purification
Hepatitis C Antibodies / immunology*
Humans
Mice
Molecular Sequence Data
Recombinant Fusion Proteins / genetics,  immunology*
Viral Proteins / genetics,  immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/HVR1 protein, Hepatitis C virus; 0/Hepatitis C Antibodies; 0/Recombinant Fusion Proteins; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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