Document Detail


Chimeric microbial rhodopsins containing the third cytoplasmic loop of bovine rhodopsin.
MedLine Citation:
PMID:  21504723     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
G-protein-coupled receptors transmit stimuli (light, taste, hormone, neurotransmitter, etc.) to the intracellular signaling systems, and rhodopsin (Rh) is the most-studied G-protein-coupled receptor. Rh possesses an 11-cis retinal as the chromophore, and 11-cis to all-trans photoisomerization leads to the protein structural changes in the cytoplasmic loops to activate G-protein. Microbial rhodopsins are similar heptahelical membrane proteins that function as bacterial sensors, light-driven ion-pumps, or light-gated channels. Microbial rhodopsins possess an all-trans retinal, and all-trans to 13-cis photoisomerization triggers protein structural changes for each function. Despite these similarities, there is no sequence homology between visual and microbial rhodopsins, and microbial rhodopsins do not activate G-proteins. However, it was reported that bacteriorhodopsin (BR) chimeras containing the third cytoplasmic loop of bovine Rh are able to activate G-protein, suggesting a common mechanism of protein structural changes. Here we design chimeric proteins for Natronomonas pharaonis sensory rhodopsin II (SRII, also called pharaonis phoborhodopsin), which has a two-orders-of-magnitude slower photocycle than BR. Light-dependent transducin activation was observed for most of the nine SRII chimeras containing the third cytoplasmic loop of bovine Rh (from Y223, G224, Q225 to T251, R252, and M253), but the activation level was 30,000-140,000 times lower than that of bovine Rh. The BR chimera, BR/Rh223-253, activates a G-protein transducin, whereas the activation level was 37,000 times lower than that of bovine Rh. We interpret the low activation by the chimeric proteins as reasonable, because bovine Rh must have been optimized for activating a G-protein transducin during its evolution. On the other hand, similar activation level of the SRII and BR chimeras suggests that the lifetime of the M intermediates is not the simple determinant of activation, because SRII chimeras have two-orders-of-magnitude's slower photocycle than the BR chimera. Activation mechanism of visual and microbial rhodopsins is discussed on the basis of these results.
Authors:
Aya Nakatsuma; Takahiro Yamashita; Kengo Sasaki; Akira Kawanabe; Keiichi Inoue; Yuji Furutani; Yoshinori Shichida; Hideki Kandori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biophysical journal     Volume:  100     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-20     Completed Date:  2011-08-11     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1874-82     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Frontier Materials, Nagoya Institute of Technology, Nagoya, Japan.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Amino Acid Sequence
Animals
Bacteriorhodopsins / chemistry*,  genetics,  metabolism*
Cattle
Cytoplasm / metabolism*
GTP-Binding Proteins / metabolism
Halobacteriaceae
Molecular Sequence Data
Photochemical Processes
Recombinant Fusion Proteins / chemistry*,  genetics,  metabolism*
Rhodopsin / chemistry*,  genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Recombinant Fusion Proteins; 53026-44-1/Bacteriorhodopsins; 9009-81-8/Rhodopsin; EC 3.6.1.-/GTP-Binding Proteins
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