Document Detail


Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D structure.
MedLine Citation:
PMID:  20939005     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Avidin and its bacterial analog streptavidin have been widely used in applications in life sciences. Recently, we described a highly thermostable engineered avidin, called chimeric avidin, which is a hybrid of avidin and avidin-related protein 4. Here, we report a protocol for pilot-scale production in E. coli and the X-ray structure of chimeric avidin. The ligand-binding properties of chimeric avidin were explored with isothermal titration calorimetry. We found chimeric avidin to be more stable against various harsh organic solvents at elevated temperatures compared to avidin and streptavidin. The properties of chimeric avidin make it a potential tool for new applications in biotechnology. Biotechnol. Bioeng. 2011; 108:481-490. © 2010 Wiley Periodicals, Inc.
Authors:
Juha A E Määttä; Yael Eisenberg-Domovich; Henri R Nordlund; Ruchama Hayouka; Markku S Kulomaa; Oded Livnah; Vesa P Hytönen
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Publication Detail:
Type:  Journal Article     Date:  2010-11-24
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  108     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-01-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  481-90     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley Periodicals, Inc.
Affiliation:
Institute of Medical Technology, Biokatu 6, FI-33014 University of Tampere and Tampere University Hospital, Tampere, Finland; telephone: +358-40-190-1517; fax: +358-3-3551-7710.
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