Document Detail


Chimeras of herpes simplex viral VP16 and jun are oncogenic.
MedLine Citation:
PMID:  8241024     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Jun protein binds DNA and regulates transcription as a component of the AP-1 transcription factor complex. In its oncogenic form, Jun can transform cells in culture and cause tumors in animals. Both trans-activation and transformation require several functional domains of Jun, including an amino-terminal trans-activation domain. In this study, properties of Jun required for trans-activation and transformation were explored by replacing the trans-activation domains of c-Jun and its oncogenic counterpart, v-Jun, with the constitutively active trans-activation domain from the herpes simplex virus VP16 protein. The VP16-v-Jun chimera retained similar oncogenic properties to its parent, v-Jun. The VP16-c-Jun chimera, however, was considerably more oncogenic than c-Jun. Substitutions of a phenylalanine in the VP16 domain of the VP16-c-Jun chimera diminished or abolished transformation. Each of the chimeras bound to the AP-1 consensus recognition sequence from the collagenase promoter or from the human T-cell leukemia virus type I long terminal repeat in vitro. None of the VP16-Jun chimeras efficiently stimulated transcription from the collagenase promoter or an artificial promoter containing the human T-cell leukemia virus type I element in vivo. These results demonstrate that the Jun trans-activation domain can be replaced by a heterologous trans-activation domain with retention of oncogenic activity. However, this oncogenic activity is not reflected in the trans-activating properties of the chimeras.
Authors:
E R Schuur; E J Parker; P K Vogt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  4     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-12-23     Completed Date:  1993-12-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  761-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Southern California School of Medicine, Los Angeles.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Line
Chick Embryo
DNA-Binding Proteins / metabolism
Herpes Simplex Virus Protein Vmw65 / genetics*
Molecular Sequence Data
Oncogene Protein p65(gag-jun) / genetics*
Recombinant Fusion Proteins / genetics*
Simplexvirus*
Transcriptional Activation*
Grant Support
ID/Acronym/Agency:
CA 42564/CA/NCI NIH HHS; F32CA09294/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Herpes Simplex Virus Protein Vmw65; 0/Oncogene Protein p65(gag-jun); 0/Recombinant Fusion Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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