Document Detail

Chimaerism following allogeneic bone marrow transplantation: detection of residual host cells using the polymerase chain reaction.
MedLine Citation:
PMID:  2818939     Owner:  NLM     Status:  MEDLINE    
Chimaerism was assessed in five recipients following sex mismatched allogeneic bone marrow transplantation. Techniques included karyotyping of bone marrow cells, dot blot DNA analysis of blood and bone marrow suspensions, and in vitro amplification of DNA by the polymerase chain reaction (PCR) using blood and bone marrow suspensions and stored bone marrow slides. Results of karyotypic analysis suggested complete chimaerism in four patients, while in one patient mixed chimaerism was detected. Mixed chimaerism was also detected, however, in a second patient using PCR and confirmed by dot blot analysis on all tissues examined. PCR is a sensitive tool for investigation of chimaerism following bone marrow transplantation. Since this technique does not require radioactivity, it is an attractive method for use in a clinical laboratory. This technique represents a further development in the use of DNA methodologies in the assessment of haematological disease.
M Lawler; S R McCann; E Conneally; P Humphries
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of haematology     Volume:  73     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1989-12-29     Completed Date:  1989-12-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  205-10     Citation Subset:  IM    
Department of Genetics, Trinity College Dublin, Ireland.
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MeSH Terms
Blotting, Southern
Bone Marrow / pathology
Bone Marrow Transplantation*
Electrophoresis, Agar Gel
Nucleic Acid Amplification Techniques*
Polymerase Chain Reaction
Sex Chromosomes
Transplantation, Homologous

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