Document Detail


Children with untreated food allergy express a relative increment in the density of duodenal gammadelta+ T cells.
MedLine Citation:
PMID:  11145283     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: We investigated whether children with food allergy (FA) express increased densities of intraepithelial gammadelta+ T cells similarly to subjects with celiac disease. METHODS: The duodenal specimens taken by gastroduodenoscopy from 20 children with untreated FA, 17 with treated FA, 12 with celiac disease (CD) and 12 controls were studied with monoclonal antibodies and a three-layer peroxidase staining method. RESULTS: The subjects with untreated FA expressed equal densities of total intraepithelial CD3+ and alpha/beta+ T cells, but significantly higher densities of gammadelta+ cells than the subjects currently on an elimination diet for FA or the controls. Accordingly, their gammadelta+/CD3+ ratio was higher. On the other hand, the results differed clearly from CD, where all the three cell populations showed high densities. Another finding that discriminated the subjects with FA from the CD patients was endoscopic examination. Lymphonodular hyperplasia (LNH) of the duodenal bulb with a normal villous pattern was demonstrated in 14 (70%) of the 20 subjects with untreated FA and in 8 (47%) of the 17 with treated FA, but in none of the celiac patients or controls. Surprisingly, the biopsy samples from the subjects with FA showed quite normal histological findings. CONCLUSIONS: According to this preliminary observation, high densities of intraepithelial gammadelta+ T cells and a significantly elevated gammadelta+/CD3+ ratio are associated with untreated FA. If seen LNH in a gastroduodenoscopy and/or increased densities of gammadelta+ T cells in the biopsy specimen, the possibility of gastrointestinal FA should be reliably assessed by a food challenge.
Authors:
J Kokkonen; K Holm; T J Karttunen; M Mäki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of gastroenterology     Volume:  35     ISSN:  0036-5521     ISO Abbreviation:  Scand. J. Gastroenterol.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-28     Completed Date:  2001-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060105     Medline TA:  Scand J Gastroenterol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  1137-42     Citation Subset:  IM    
Affiliation:
Dept. of Pediatrics, University of Oulu, Finland.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Antigens, CD3 / analysis
Celiac Disease / immunology*,  pathology
Child
Child, Preschool
Duodenum / immunology*,  pathology
Endoscopy, Gastrointestinal
Female
Food Hypersensitivity / immunology*,  pathology,  therapy
HLA-DR Antigens / analysis
Humans
Immunohistochemistry
Infant
Intestinal Mucosa / immunology*,  pathology
Lymphocyte Count
Male
Receptors, Antigen, T-Cell, alpha-beta / analysis
Receptors, Antigen, T-Cell, gamma-delta / analysis*
T-Lymphocyte Subsets*
Chemical
Reg. No./Substance:
0/Antigens, CD3; 0/HLA-DR Antigens; 0/Receptors, Antigen, T-Cell, alpha-beta; 0/Receptors, Antigen, T-Cell, gamma-delta

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