| Children with celiac disease express inducible nitric oxide synthase in the small intestine during gluten challenge. | |
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MedLine Citation:
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PMID: 9759949 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Childhood celiac disease in Sweden is presently seen at an incidence of around 1/250 and is thus one of the commonest chronic diseases in children. It has recently been shown that children with untreated celiac disease have increased levels of nitrate/nitrite in the urine, most likely reflecting an increased production of nitric oxide in the inflamed mucosa. Nitric oxide is produced from L-arginine by an inducible or a constitutive nitric oxide synthase. The inducible nitric oxide synthase (iNOS) can be stimulated in various cells by, for instance, inflammatory mediators. The present study has been done to find a possible source of nitric oxide in the small intestine that could result in the increased levels of nitrate/nitrite in the urine in children with active celiac disease. METHODS: Small-intestinal biopsy specimens from children with active celiac disease were labeled with rabbit-anti-human antibodies to iNOS and visualized with fluorescent pig anti-rabbit antibodies. The specimens were then analyzed with confocal microscopy to assess the labeling pattern. RESULTS: In all of seven specimens from children with increased levels of nitrate/nitrite in the urine, we detected antibodies to iNOS, whereas in five of six control specimens--that is, from children with normal nitrate/nitrite levels--we could not detect any iNOS. CONCLUSIONS: Children with active celiac disease have a gluten-induced nitric oxide production in the small intestine reflected by increased urine levels of nitrate/nitrite and iNOS expression in the intestine. We conclude that the increased production of nitric oxide could presumably, directly or indirectly, result in injury of the small-intestinal tissue. |
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Authors:
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K Holmgren Peterson; K Fälth-Magnusson; K E Magnusson; L Stenhammar; T Sundqvist |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of gastroenterology Volume: 33 ISSN: 0036-5521 ISO Abbreviation: Scand. J. Gastroenterol. Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1998-12-16 Completed Date: 1998-12-16 Revised Date: 2011-10-27 |
Medline Journal Info:
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Nlm Unique ID: 0060105 Medline TA: Scand J Gastroenterol Country: NORWAY |
Other Details:
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Languages: eng Pagination: 939-43 Citation Subset: IM |
Affiliation:
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Dept. of Medical Microbiology, Linköping University, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Celiac Disease / enzymology* Child, Preschool Glutens / pharmacology* Humans Immunohistochemistry Infant Intestine, Small / enzymology* Microscopy, Confocal Nitrates / urine Nitric Oxide / biosynthesis Nitric Oxide Synthase / analysis* Nitric Oxide Synthase Type II Nitrites / urine |
| Chemical | |
Reg. No./Substance:
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0/Nitrates; 0/Nitrites; 10102-43-9/Nitric Oxide; 8002-80-0/Glutens; EC 1.14.13.39/NOS2 protein, human; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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