Document Detail


Child cerebrospinal fluid analysis by capillary electrophoresis and laser-induced fluorescence detection.
MedLine Citation:
PMID:  8520681     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was used to analyze a 50-microliters sample of cerebrospinal fluid from leukaemic children treated with high doses of methotrexate. Free amino acids and primary amines are labelled with fluorescein isothiocyanate prior to analysis. Electropherograms containing more than 50 peaks were obtained in less than 22 min. Twenty-one peaks were identified, and 19 were quantitated. Observed differences in individual amino acid levels are compared with healthy reference values. The results indicate that CE-LIF is useful as a selective, rapid and sensitive tool for the determination of free amino acids and amines in clinical biology studies.
Authors:
G Nouadje; H Rubie; E Chatelut; P Canal; M Nertz; P Puig; F Couderc
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  717     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-01-25     Completed Date:  1996-01-25     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  293-8     Citation Subset:  IM    
Affiliation:
ZETA Technology, Parc Tecnologique du Canal, Toulouse, France.
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MeSH Terms
Descriptor/Qualifier:
6-Mercaptopurine / administration & dosage
Adolescent
Amines / cerebrospinal fluid
Amino Acids / cerebrospinal fluid
Antineoplastic Combined Chemotherapy Protocols / therapeutic use
Child
Cytarabine / administration & dosage
Electrophoresis, Capillary / methods*
Female
Humans
Lasers
Male
Methotrexate / administration & dosage
Precursor Cell Lymphoblastic Leukemia-Lymphoma / cerebrospinal fluid*,  drug therapy
Spectrometry, Fluorescence / methods*
Chemical
Reg. No./Substance:
0/Amines; 0/Amino Acids; 147-94-4/Cytarabine; 50-44-2/6-Mercaptopurine; 59-05-2/Methotrexate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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