| Child cerebrospinal fluid analysis by capillary electrophoresis and laser-induced fluorescence detection. | |
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MedLine Citation:
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PMID: 8520681 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was used to analyze a 50-microliters sample of cerebrospinal fluid from leukaemic children treated with high doses of methotrexate. Free amino acids and primary amines are labelled with fluorescein isothiocyanate prior to analysis. Electropherograms containing more than 50 peaks were obtained in less than 22 min. Twenty-one peaks were identified, and 19 were quantitated. Observed differences in individual amino acid levels are compared with healthy reference values. The results indicate that CE-LIF is useful as a selective, rapid and sensitive tool for the determination of free amino acids and amines in clinical biology studies. |
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Authors:
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G Nouadje; H Rubie; E Chatelut; P Canal; M Nertz; P Puig; F Couderc |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. A Volume: 717 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 1995 Nov |
Date Detail:
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Created Date: 1996-01-25 Completed Date: 1996-01-25 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 293-8 Citation Subset: IM |
Affiliation:
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ZETA Technology, Parc Tecnologique du Canal, Toulouse, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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6-Mercaptopurine
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administration & dosage Adolescent Amines / cerebrospinal fluid Amino Acids / cerebrospinal fluid Antineoplastic Combined Chemotherapy Protocols / therapeutic use Child Cytarabine / administration & dosage Electrophoresis, Capillary / methods* Female Humans Lasers Male Methotrexate / administration & dosage Precursor Cell Lymphoblastic Leukemia-Lymphoma / cerebrospinal fluid*, drug therapy Spectrometry, Fluorescence / methods* |
| Chemical | |
Reg. No./Substance:
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0/Amines; 0/Amino Acids; 147-94-4/Cytarabine; 50-44-2/6-Mercaptopurine; 59-05-2/Methotrexate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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