| Chicken perinatal troponin Ts are generated by a combination of novel and phylogenetically conserved alternative splicing pathways. | |
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MedLine Citation:
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PMID: 7556899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During perinatal development, avian and mammalian skeletal muscles express a novel set of troponin T (TnT) isoforms with higher M(r)s and more acidic pIs than their adult counterparts. In mammals, these TnTs result from the incorporation of a developmentally regulated 5'-fetal exon into fast TnT mRNAs. To determine whether chicken perinatal TnTs are generated by a similar mechanism, we sequenced 40 chicken TnT 5'-cDNAs from perinatal and adult pectoralis. Evidence for five 5'-exons not present in the mammalian gene was found, including one, y, whose splicing is developmentally regulated. Although none of these new exons are homologous with the mammalian fetal exon, their alternative splicing, superimposed on three conserved splicing patterns of the phylogenetically shared 5'-exons, generates the chicken perinatal TnT isoforms. These observations indicate that chicken, and most likely other avian species, evolved an independent 5'-alternative splicing mechanism to generate perinatal TnTs that have the same biophysical, and presumably functional, properties as their mammalian homologs. |
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Authors:
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F Schachat; J M Schmidt; M Maready; M M Briggs |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Developmental biology Volume: 171 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 1995 Sep |
Date Detail:
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Created Date: 1995-10-30 Completed Date: 1995-10-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 233-9 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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genetics Alternative Splicing* Amino Acid Sequence Animals Base Sequence Chick Embryo Chickens DNA Primers DNA, Complementary Exons Molecular Sequence Data Muscle Development Muscles / embryology, metabolism Phylogeny Troponin / genetics* Troponin T |
| Grant Support | |
ID/Acronym/Agency:
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AR 39603/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Complementary; 0/Troponin; 0/Troponin T |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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