| Chicken egg antibodies for immunohistochemical labeling of growth hormone and prolactin in bovine pituitary gland. | |
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MedLine Citation:
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PMID: 8354884 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe the production of polyclonal chicken antibodies specific for bovine growth hormone (bGH) and prolactin (PRL). Antibodies were generated by immunization of laying hens with recombinant bGH (rbGH), pituitary derived bGH (pbGH), and ovine PRL (oPRL). After the lipoprotein fraction was removed by dextran sulfate precipitation the antibodies were isolated from the egg yolks by ammonium sulfate precipitation. Immunization with rbGH and oPRL generated large amounts of specific antibodies, as revealed by ELISA and Western blot analysis. Antibodies against pbGH showed pronounced crossreactions with oPRL. The antibodies against rbGH and oPRL were well suited for sensitive and specific labeling of the GH- and PRL-synthesizing cells in bovine pituitary glands by immunohistochemistry. In addition, a quick and sensitive procedure for demonstration of both bGH- and PRL-synthesizing cells in a single paraffin section by double immunohistochemistry is presented. The chicken anti-bGH antibodies showed excellent results in combination with rabbit anti-PRL antibodies. The main advantage of avian antibodies in double immunostaining methods is the lack of crossreactions between avian antibodies and mammalian immunoglobulins and receptors which bind to the crystalline fragment of mammalian immunoglobulins (Fc receptors). |
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Authors:
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P Schmidt; M H Erhard; D Schams; A Hafner; S Folger; U Lösch |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 41 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1993-09-21 Completed Date: 1993-09-21 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1441-6 Citation Subset: IM |
Affiliation:
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Institutes of Veterinary Pathology, Ludwig Maximilians University, Munich, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / analysis*, immunology Blotting, Western Cattle Chickens / immunology Egg Proteins / immunology* Egg Yolk / immunology Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Growth Hormone / analysis*, immunology Immunoglobulin G / analysis, immunology Immunohistochemistry / methods Pituitary Gland / chemistry*, cytology, immunology Prolactin / analysis*, immunology Sheep |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Egg Proteins; 0/Immunoglobulin G; 9002-62-4/Prolactin; 9002-72-6/Growth Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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