| ChickGCE: a novel germ cell EST database for studying the early developmental stage in chickens. | |
| | |
MedLine Citation:
|
PMID: 16714094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We established a database to study germ cells during the early developmental stage in the chicken. The ChickGCE database provides integrated expressed sequence tag (EST) data from chicken testis, ovary, embryonic gonads, and primordial germ cells. We gathered data on 10,294 ESTs from approximately 1000 embryonic gonads, and we experimentally determined 10,851 ESTs from primordial germ cells purified from 7955 embryonic gonads by magnetically activated cell sorting. The EST testis and ovary datasets were retrieved from the public database of The Institute for Genomic Research (TIGR). The EST data were clustered and assembled into unique sequences, contigs, and singletons. The ChickGCE database provides functional annotation, identification, and putative embryonic germ-cell-specific novel transcripts based on the Gene Ontology database, as well as statistical analyses of expression patterns and pair-wise comparisons of two types of tissue- and germ-cell-specific alternative splicing events in the chicken. The new database is accessible online and queries can be answered using several search options, including tissue database searches, keywords, clone IDs, expected values, and BLAST search scores. |
| | |
Authors:
|
Heebal Kim; Dajeong Lim; Beom Ku Han; Samsun Sung; Mina Jeon; Sunjin Moon; Yeonkyung Kang; Jungrye Nam; Jae Yong Han |
Related Documents
:
|
12915734 - Gamma-tubulin function during female germ-cell development and oogenesis in drosophila. 1289064 - Characterization of a germ-line proliferation mutation in c. elegans. 12130804 - The germ cell lineage identified by vas-mrna during the embryogenesis in goldfish. 9810494 - Characterisation of cytotrophoblastic-like cells present in subinvolutioned placental s... 21487924 - Separation and viability of gill and hepatopancreatic cells of a mangrove crab ucides c... 6445034 - Mutagenesis of ultraviolet-irradiated lambda phage by host cell irradiation: induction ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-22 |
Journal Detail:
|
Title: Genomics Volume: 88 ISSN: 0888-7543 ISO Abbreviation: Genomics Publication Date: 2006 Aug |
Date Detail:
|
Created Date: 2006-07-21 Completed Date: 2006-11-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8800135 Medline TA: Genomics Country: United States |
Other Details:
|
Languages: eng Pagination: 252-7 Citation Subset: IM |
Affiliation:
|
Department of Food and Animal Biotechnology, Seoul National University, Seoul 151-742, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chick Embryo / metabolism* Chickens / genetics* Computational Biology Databases, Nucleic Acid* Expressed Sequence Tags* Gene Expression Germ Cells / metabolism* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Identification and characterization of RTVP1/GLIPR1-like genes, a novel p53 target gene cluster.
Next Document: Identification of a unique splice site variant in SLC39A4 in bovine hereditary zinc deficiency, leth...