| Chemotaxonomy of bacteria by comprehensive GC and GC-MS in electron impact and chemical ionisation mode. | |
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MedLine Citation:
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PMID: 18792008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The analysis of the cellular lipidic fraction of bacteria is described. After hydrolysis and methylation, the fatty acid methyl esters (FAMEs) are determined by 1-D GC using the Sherlock MIDI bacteria identification system, by comprehensive GC (GC x GC) and by GC-MS in electron impact (EI) and positive chemical ionisation (PCI) mode. With GC x GC, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. GC-EI-MS and GC-PCI-MS were helpful for confirmation. The bacteria selected in this study are Brevundimonas diminuta, Chryseobacterium gleum and Stenotrophomonas maltophilia. |
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Authors:
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Frank David; Bart Tienpont; Pat Sandra |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of separation science Volume: 31 ISSN: 1615-9314 ISO Abbreviation: J Sep Sci Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-22 Completed Date: 2008-12-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: Germany |
Other Details:
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Languages: eng Pagination: 3395-403 Citation Subset: IM |
Affiliation:
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Research Institute for Chromatography, Kortrijk, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteria / chemistry*, classification Chromatography, Gas / instrumentation, methods* Gas Chromatography-Mass Spectrometry / instrumentation, methods* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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