Document Detail


Chemotaxonomy of bacteria by comprehensive GC and GC-MS in electron impact and chemical ionisation mode.
MedLine Citation:
PMID:  18792008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The analysis of the cellular lipidic fraction of bacteria is described. After hydrolysis and methylation, the fatty acid methyl esters (FAMEs) are determined by 1-D GC using the Sherlock MIDI bacteria identification system, by comprehensive GC (GC x GC) and by GC-MS in electron impact (EI) and positive chemical ionisation (PCI) mode. With GC x GC, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. GC-EI-MS and GC-PCI-MS were helpful for confirmation. The bacteria selected in this study are Brevundimonas diminuta, Chryseobacterium gleum and Stenotrophomonas maltophilia.
Authors:
Frank David; Bart Tienpont; Pat Sandra
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of separation science     Volume:  31     ISSN:  1615-9314     ISO Abbreviation:  J Sep Sci     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-22     Completed Date:  2008-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3395-403     Citation Subset:  IM    
Affiliation:
Research Institute for Chromatography, Kortrijk, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / chemistry*,  classification
Chromatography, Gas / instrumentation,  methods*
Gas Chromatography-Mass Spectrometry / instrumentation,  methods*

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