| Chemotaxis of alveolar macrophages in response to signals derived from alveolar epithelial cells. | |
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MedLine Citation:
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PMID: 9605106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have postulated that alveolar epithelial cells (AEC) play a critical role in local regulation of alveolar macrophage (AM) recruitment and activation for host defense in the lung. The present study explores the effects of conditioned medium from AEC (AEC-CM) on the migration of AM, using a Boyden chamber assay. AEC-CM was chemotactic for AM, with peak activity observed with a 1:10 dilution. We previously showed that rat AEC express the chemokines RANTES (regulated on activation, normal T expressed and secreted) and monocyte chemoattractant protein 1 (MCP-1) as well as granulocyte-macrophage colony-stimulating factor (GM-CSF). Neutralizing antibodies to RANTES and to MCP-1 and immunoprecipitation of GM-CSF decreased the chemotactic activity of AEC-CM by 58%, 29%, and 47%, respectively. Similar levels of chemotaxis were found in response to recombinant RANTES, MCP-1, and GM-CSF. In each instance the optimal dose was very low (0.01 to 0.1 ng/ml), with diminished chemotaxis at higher doses. Peritoneal macrophages (PM) also migrated in response to AEC-CM and each of the recombinant cytokines; however, AM were much more sensitive to AEC-CM, RANTES, and GM-CSF than were PM. AM migrated preferentially from medium conditioned by unstimulated AEC toward supernatants from interleukin 1alpha-stimulated AEC. Therefore, AEC may control the distribution of AM through the creation of local chemotactic gradients and are likely to play a critical role in the host response to low-level antigen entry into the peripheral lung. |
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Authors:
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A D O'Brien; T J Standiford; P J Christensen; S E Wilcoxen; R Paine |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of laboratory and clinical medicine Volume: 131 ISSN: 0022-2143 ISO Abbreviation: J. Lab. Clin. Med. Publication Date: 1998 May |
Date Detail:
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Created Date: 1998-06-19 Completed Date: 1998-06-19 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375375 Medline TA: J Lab Clin Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 417-24 Citation Subset: AIM; IM |
Affiliation:
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Department of Internal Medicine, University of Michigan, and the Veterans Affairs Medical Center, Ann Arbor, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / immunology, pharmacology Cell Movement / physiology Chemokine CCL2 / immunology, pharmacology Chemokine CCL5 / immunology, pharmacology Chemotactic Factors / pharmacology Chemotaxis / drug effects, physiology* Culture Media, Conditioned / pharmacology Epithelial Cells / physiology* Granulocyte-Macrophage Colony-Stimulating Factor / immunology, physiology Macrophages, Alveolar / drug effects, physiology* Macrophages, Peritoneal / drug effects Male Pulmonary Alveoli / cytology, physiology* Rats Rats, Sprague-Dawley Signal Transduction* / physiology |
| Grant Support | |
ID/Acronym/Agency:
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1P50HL46487/HL/NHLBI NIH HHS; HL50496/HL/NHLBI NIH HHS; HL58200/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Chemokine CCL2; 0/Chemokine CCL5; 0/Chemotactic Factors; 0/Culture Media, Conditioned; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor |
| Comments/Corrections | |
Comment In:
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J Lab Clin Med. 1998 May;131(5):391-2
[PMID:
9605102
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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