| Chemotactic responses of normal human basophils to C5a and to lymphocyte-derived chemotactic factor. | |
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MedLine Citation:
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PMID: 932427 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peripheral blood basophils from normal human subjects responded chemotactically to the complement component C5a and also to the supernatant fluid from cultured human lymphocytes stimulated by mitogens or specific antigen. Basophil chemotactic factor (BCF) was detected in stimulated lymphocyte cultures within 24 hr and increased for the first 3 days. BCF was found after stimulation of lymphocytes by B or T cell mitogens as well as by specific antigen. The amount of BCF in culture fluids was independent of cell proliferation. Monocyte chemotactic factor (MCF) and BCF could not be separated from one another by Sephadex or DEAE chromatography. Both activities were eluted from Sephadex G-100 columns in a m.w. region of about 15,000 daltons and were found after ion exchange chromatography on DEAE in a peak eluted by 0.1 M NaCl. These findings suggest that mitogen or antigen-stimulated lymphocytes secrete a molecule that is chemotactic for both monocytes and basophils. Thus, in the absence of a basophil-specific chemotactic molecule, the preferential accumulation of basophils in certain types of delayed hypersensitivity skin reactions must be due to additional factors such as basophil chemotaxis augmentation factor. |
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Authors:
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M A Lett-Brown; D A Boetcher; E J Leonard |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 117 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1976 Jul |
Date Detail:
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Created Date: 1976-08-23 Completed Date: 1976-08-23 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 246-52 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Basophils
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immunology Cell-Free System Chemotaxis* Complement C5 / metabolism* Complement System Proteins / metabolism* Humans Lymphocytes / immunology* Monocytes / immunology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Complement C5; 9007-36-7/Complement System Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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